{"text_format":"1","workbook":"xlsx","n_sheets":20,"sheets":["Readme","List Manager","Tested Thermal Cyclers","Tested Thermal Cyclers ","Gene Panels","FFPE Kits & Addtl Reagents","FF Kits & Addtl Reagents","Assay Steps & Timing","Pre-run QC","Xenium v1 Workflows","Xenium Protein Workflow","Xenium Prime Workflow","Xenium Analyzer","Assay Kits & Reagents","Instrument Kits & Reagents","Post-run H&E Kits & Reagents","Post-run IF Kits & Reagents","csv for Connect","WorksheetWillHide","Sheet3"],"not_rendered":{"charts":0,"images":5,"macros":1,"external_links":1,"defined_names":41,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"Readme","index":0,"n_rows":62,"n_cols":17,"rows_kept":62,"cut":false,"hidden":false,"merged":[[9,1,9,12],[8,1,8,12],[44,1,44,2],[44,3,44,8],[26,1,26,11],[27,0,27,12],[28,0,28,12],[29,1,29,12],[30,3,30,4],[30,1,30,2],[32,1,32,2],[33,1,37,2],[38,1,39,2],[40,1,43,2],[37,3,37,8],[37,9,37,12],[44,9,44,12],[39,3,39,8],[4,0,4,12],[5,0,5,12],[6,1,6,12],[7,1,7,12],[38,3,38,8],[38,9,38,12],[10,1,10,12],[11,1,11,12],[12,1,12,12],[13,0,13,12],[14,0,14,12],[36,3,36,8],[36,9,36,12],[35,3,35,8],[17,1,17,11],[21,1,21,11],[25,1,25,11],[16,1,16,9],[20,1,20,9],[0,0,0,12],[0,13,3,13],[1,0,2,1],[1,2,1,11],[2,2,2,11],[3,0,3,12],[58,1,58,12],[59,1,59,12],[60,1,60,12],[61,1,61,12],[62,1,62,12],[50,0,50,12],[51,3,51,12],[55,1,55,12],[56,1,56,12],[57,0,57,12],[52,3,52,12],[53,1,53,12],[54,1,54,12],[46,3,46,8],[46,9,46,12],[47,1,47,12],[48,1,48,12],[49,1,49,12],[45,1,46,2],[45,3,45,8],[45,9,45,12],[43,3,43,8],[43,9,43,12],[32,9,32,12],[33,3,33,8],[33,9,33,12],[34,3,34,8],[34,9,34,12],[32,3,32,8],[39,9,39,12],[40,3,40,8],[35,9,35,12],[24,1,24,9],[42,3,42,8],[42,9,42,12],[40,9,40,12],[41,3,41,8],[41,9,41,12],[30,6,30,7],[30,8,30,9],[30,10,30,12],[31,1,31,12]]}
[]
[null,null,"PROTOCOL PLANNER",null,null,null,null,null,null,null,null,null,"CG000601"]
[null,null,"Xenium In Situ Gene Expression",null,null,null,null,null,null,null,null,null,"Rev G"]
[]
[]
["Introduction"]
[null,"Xenium In Situ measures gene expression in tissue sections derived from either formalin fixed and paraffin embedded (FFPE) or fresh frozen (FF) tissue samples placed on Xenium Slides. This Protocol Planner provides an overview of Xenium In Situ (refers to Xenium v1 workflows - Gene Expression, Cell Segmentation), Xenium Protein (refers to Xenium Protein workflow - Gene Expression, Protein Expression, Cell Segmentation), and Xenium Prime (refers to Xenium Prime workflow - Gene Expression, Cell Segmentation). To enable efficient planning, a breakdown of key protocol steps and times, list of user-acquired reagents and consumables, and information about supporting documentation that will be available for executing the Xenium workflows is also provided."]
[null,"Reagents and hardware produced by 10x Genomics are not listed in this document. Unless otherwise indicated, reagent and equipment tables apply to all assays."]
[]
[null,"Use Guidelines:\nThis file is best viewed using Microsoft Excel. Opening the file in another program may break document features. \nThis file contains macros to highlight some key workflow specifics. Enable macros before using this document. Windows users: Right-click on the file, select \"Properties\". In the Properties window select \"Unblock\" under Security section. Mac users: Open the file, and when prompted, select \"Enable Macros\""]
[null,"Copy-Paste Guidelines:\nThe sheet is protected to prevent altering the content. In order to copy-paste, right click to select a cell's text."]
[null,"Printing Guidelines:\nThe print area has been set for each tab in this document. Go to Print. For orientation, select \"Portrait.\" For scaling, select \"Fit all columns to one page.\" \nTo print the entire workbook, Go to Print and under settings select \"Print Entire Workbook.\" \nTo create a pdf document, go to Print and select \"Adobe PDF\" as the printer. Do not go to \"Save as Adobe PDF.\""]
[]
["Workflow Overview"]
["Xenium In Situ (v1)"]
[null,"Xenium In Situ Gene Expression Workflow AND Xenium In Situ Gene Expression with Cell Segmentation Staining Workflow"]
[]
[null,"Figure 1. Xenium v1 workflow overview"]
["Xenium Protein"]
[null,"Xenium In Situ Gene and Protein Expression with Cell Segmentation Staining Workflow"]
[]
[null,"Figure 2. Xenium Protein workflow overview."]
["Xenium Prime"]
[null,"Xenium Prime In Situ Gene Expression with optional Cell Segmentation Staining Workflow"]
[]
[null,"Figure 3. Xenium Prime with optional Cell Segmentation workflow overview."]
[]
["Document Resources"]
["Xenium In Situ Gene Expression"]
[null,"Select assay and tissue preservation method. The relevant handbook and assay user guide will be highlighted"]
[null,"Select one assay:",null,"Xenium_Protein",null,"and","Select one tissue preservation method:",null,"FFPE",null,"Xenium Protein is compatible\nwith FFPE tissue only"]
[]
[null,"Steps",null,"Document",null,null,null,null,null,"Section on 10x Genomics Support Website & Doc Number"]
[1,"Experimental Design & Planning\nBefore starting the experiment, refer to these documents for protocol planning and preparation.",null,"Xenium In Situ Protocol Planner\nWorkflow overview and additional reagents for all Xenium assays",null,null,null,null,null,"User Guide\nCG000601"]
[null,null,null,"Xenium Workflow Planning Guide\nKey considerations for the various design and execution steps of the Xenium In Situ Gene Expression workflow",null,null,null,null,null,"Technical Note\nCG000754"]
[null,null,null,"Supported Thermal Cyclers\nSupported thermal cyclers for Xenium workflows",null,null,null,null,null,"Supported Thermal Cyclers for Xenium"]
[null,null,null,"Xenium Panel Designer\nInformation regarding pre-designed panels and how to request custom panel designs",null,null,null,null,null,"Xenium Panel Designer Support Page"]
[null,null,null,"Xenium Add-on Panel Design\nGuidance for selecting genes to include on Xenium add-on panels",null,null,null,null,null,"Technical Note\nCG000643"]
[2,"Tissue Slide & Sample Preparation\nPrepare tissue slides. Choose one protocol. ",null,"Xenium In Situ - FFPE Tissue Preparation Handbook\nSection FFPE tissue onto Xenium Slides, deparaffinize and decrosslink FFPE tissue sections",null,null,null,null,null,"Handbook\nCG000578",null,null,null,null,null,{"$f":"IF(OR(D31=\"Xenium_v1\",D31=\"Xenium_v1_Cell_Seg\",D31=\"Xenium_Prime\",D31=\"Xenium_Protein\"),\"Y\",\"N\")","v":"Y"},{"$f":"IF(I31=\"FFPE\",\"Y\",\"N\")","v":"Y"}]
[null,null,null,"Xenium In Situ - FF Tissue Preparation Handbook\nSection FF tissue onto Xenium Slides & fix and permeabilze FF tissue sections",null,null,null,null,null,"Handbook\nCG000579",null,null,null,null,null,{"$f":"IF(OR(D31=\"Xenium_v1\",D31=\"Xenium_v1_Cell_Seg\",D31=\"Xenium_Prime\",D31=\"Xenium_Protein\"),\"Y\",\"N\")","v":"Y"},{"$f":"IF(I31=\"Fresh Frozen\",\"Y\",\"N\")","v":"N"}]
[3,"Assay Workflow\nChoose one protocol.",null,"Xenium In Situ Gene Expression\nFor FFPE and FF samples on Xenium Slides",null,null,null,null,null,"User Guide\nCG000582",null,null,null,null,null,{"$f":"IF(D31=\"Xenium_v1\",\"Y\",\"N\")","v":"N"},{"$f":"IF(OR(I31=\"FFPE\",I31=\"Fresh Frozen\"),\"Y\",\"N\")","v":"Y"}]
[null,null,null,"Xenium In Situ Gene Expression with Cell Segmentation Staining\nFor FFPE and FF samples on Xenium Slides with Cell Segmentation",null,null,null,null,null,"User Guide\nCG000749",null,null,null,null,null,{"$f":"IF(D31=\"Xenium_v1_Cell_Seg\",\"Y\",\"N\")","v":"N"},{"$f":"IF(OR(I31=\"FFPE\",I31=\"Fresh Frozen\"),\"Y\",\"N\")","v":"Y"}]
[null,null,null,"Xenium Prime In Situ Gene Expression with Optional Cell Segmentation Staining\nFor FFPE and FF samples on Xenium Slides for Xenium Prime",null,null,null,null,null,"User Guide\nCG000760",null,null,null,null,null,{"$f":"IF(D31=\"Xenium_Prime\",\"Y\",\"N\")","v":"N"},{"$f":"IF(OR(I31=\"FFPE\",I31=\"Fresh Frozen\"),\"Y\",\"N\")","v":"Y"}]
[null,null,null,"Xenium In Situ Gene and Protein Expression with Cell Segmentation Staining\nFor FFPE samples on Xenium Slides for gene and protein expression with Cell Segmentation Staining",null,null,null,null,null,"User Guide \nCG000819",null,null,null,null,null,{"$f":"IF(D31=\"Xenium_Protein\",\"Y\",\"N\")","v":"Y"},{"$f":"IF(I31=\"FFPE\",\"Y\",\"N\")","v":"Y"}]
[4,"Instrument",null,"Xenium Analyzer\nSetup and run instructions for the Xenium Analyzer",null,null,null,null,null,"User Guide\nCG000584"]
[5,"Post-Instrument Activities (Optional)",null,"H&E Staining (Optional)\nH&E stain post-Xenium run",null,null,null,null,null,"Demonstrated Protocol\nCG000613"]
[null,null,null,"Post-Xenium In Situ Applications: Immunofluorescence, H&E, Visium v2, and Visium HD*\nPost-Xenium workflows on the same FFPE/FF tissue sections",null,null,null,null,null,"Technical Note\nCG000709"]
[null,"*Post-Xenium Visium v2 and Visium HD has not been validated for the Xenium In Situ Gene and Protein Expression assay. For information on Post-Xenium IF for the Xenium in Situ Gene and Protein Expression assay, see Technical Note CG000829)"]
[]
[]
["Document Revision Summary"]
[null,"Updated","Version","Summary of Changes"]
[null,"2025-08-01","Rev G","Updated format to Excel sheet\nAdded Xenium Protein workflow image and information to Readme tab\nAdded Xenium Protein compatible thermal cyclers to Tested Thermal Cyclers tab\nAdded Xenium Protein Protocol Steps & Timing and Additional Reagents to Xenium Protein Workflow tab \nUpdated references to FFPE and FF Tissue Preparation Handbooks throughout"]
[]
[]
[null,"© 2025 10x Genomics, Inc. (10x Genomics). All rights reserved. Duplication and/or reproduction of all or any portion of this document without the express written consent of 10x Genomics, is strictly forbidden.  Nothing contained herein shall constitute any warranty, express or implied, as to the performance of any products described herein. Any and all warranties applicable to any products are set forth in the applicable terms and conditions of sale accompanying the purchase of such product. 10x Genomics provides no warranty and hereby disclaims any and all warranties as to the use of any third-party products or protocols described herein. The use of products described herein is subject to certain restrictions as set forth in the applicable terms and conditions of sale accompanying the purchase of such product. A non-exhaustive list of 10x Genomics’ marks, many of which are registered in the United States and other countries can be viewed at: www.10xgenomics.com/trademarks. 10x Genomics may refer to the products or services offered by other companies by their brand name or company name solely for clarity, and does not claim any rights in those third-party marks or names. 10x Genomics products may be covered by one or more of the patents as indicated at: www.10xgenomics.com/patents. All products and services described herein are intended FOR RESEARCH USE ONLY and NOT FOR USE IN DIAGNOSTIC PROCEDURES. \n\nThe use of 10x Genomics products in practicing the methods set forth herein has not been validated by 10x Genomics, and such non-validated use is NOT COVERED BY \n10X GENOMICS STANDARD WARRANTY, AND 10X GENOMICS HEREBY DISCLAIMS ANY AND ALL WARRANTIES FOR SUCH USE. Nothing in this document should be construed \nas altering, waiving or amending in any manner 10x Genomics terms and conditions of sale for the Chromium Controller or the Chromium Single Cell Controller, consumables or software, including without limitation such terms and conditions relating to certain use restrictions, limited license, warranty and limitation of liability, and nothing in this document shall be deemed to be Documentation, as that term is set forth in such terms and conditions of sale. Nothing in this document shall be construed as any representation by 10x Genomics that it currently or will at any time in the future offer or in any way support any application set forth herein."]
[]
["Contact"]
[null,"support@10xgenomics.com"]
[null,"10x Genomics, Inc"]
[null,"6230 Stoneridge Mall Road"]
[null,"Pleasanton, CA 94588 USA"]

{"sheet":"List Manager","index":1,"n_rows":8,"n_cols":5,"rows_kept":8,"cut":false,"hidden":true,"merged":[]}
["List Options (for highlighting)"]
[]
["Assay","Xenium_v1","Xenium_v1_Cell_Seg","Xenium_Prime","Xenium_Protein"]
[]
["Xenium_v1","FFPE","FFPE","FFPE","FFPE"]
["Xenium_v1_Cell_Seg","Fresh Frozen","Fresh Frozen","Fresh Frozen"]
["Xenium_Prime"]
["Xenium_Protein"]

{"sheet":"Tested Thermal Cyclers","index":2,"n_rows":55,"n_cols":16,"rows_kept":55,"cut":false,"hidden":true,"merged":[[16,1,16,4],[17,1,17,4],[23,1,23,12],[24,1,24,12],[6,1,6,2],[7,1,7,4],[8,1,8,4],[9,1,9,4],[10,1,10,4],[11,1,11,4],[12,1,12,4],[5,1,5,12],[6,9,6,12],[13,1,13,4],[14,1,14,4],[15,1,15,4],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Tested Thermal Cyclers",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"The table below lists the thermal cyclers that have been tested by 10x Genomics for all available Xenium In Situ Gene Expression assays. Thermal cycler adaptors and compatability differ between assay workflow. Failure to use correct adaptor and thermal cycler will result in assay failure. Confirm the right adaptor and thermal cycler is used prior to starting experiment."]
[]
[null,"Thermal Cycler",null,null,null,"Part Number (US)","Xenium v1","Xenium Prime","Xenium Protein","Customer Notes"]
[null,"Analytik Jena Biometra TAdvanced 96 S †\t",null,null,null,"846-x-070-251"]
[null,"Analytik Jena Biometra TAdvanced 96 SG †\t",null,null,null,"846-x-070-241"]
[null,"Eppendorf Mastercycler X50s ‡\t",null,null,null,6311000010]
[null,"Eppendorf Mastercycler X50a ‡",null,null,null,"6313000018\t"]
[null,"BioRad PTC Tempo Deepwell\t",null,null,null,12015392]
[null,"ThermoFisher VeritiPro ¶\t",null,null,null,"A48141"]
[null,"VWR XT96 Gradient",null,null,null,"76452-153\t"]
[null,"MJ Research PTC-200 (discontinued)",null,null,null,"05434-05\t"]
[null,"BioRad C1000 (discontinued)",null,null,null,1851197]
[null,"ThermoFisher Veriti (discontinued)",null,null,null,4375786]
[null,"Eppendorf Mastercycler Pro (discontinued)",null,null,null,950030010]
[]
[null,null,"Supported"]
[null,null,"Not supported"]
[null,null,"Not tested"]
[]
[null,"† Analytik Jena Biometra TAdvanced 96 S/SG: 2°C/sec ramp rates for both heating and cooling. x in part number = 2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz\n‡ Eppendorf Mastercycler X50s/X50a: 3°C/sec ramp rate for heating and 2°C/sec ramp rate for cooling\n¶ ThermoFisher VeritiPro requires FW 1.2.0 and \"Cover Ramping\" enabled"]
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[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(OR(P4=\"FFPE – GEX\", P4=\"FFPE – PEX\"),\"Y\",\"N\")","v":"N"}]

{"sheet":"Tested Thermal Cyclers ","index":3,"n_rows":32,"n_cols":16,"rows_kept":32,"cut":false,"hidden":false,"merged":[[20,1,20,5],[20,6,20,7],[20,8,20,9],[21,1,21,5],[21,6,21,7],[21,8,21,9],[19,6,19,7],[19,8,19,9],[16,0,16,12],[18,1,18,5],[18,6,18,7],[18,8,18,9],[18,10,18,12],[19,10,19,12],[19,1,19,5],[11,10,11,12],[13,1,13,5],[14,1,14,5],[11,1,11,5],[12,1,12,5],[12,6,12,7],[12,8,12,9],[13,6,13,7],[13,8,13,9],[13,10,13,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[29,10,29,12],[34,1,34,12],[35,1,35,12],[30,1,30,5],[30,6,30,7],[30,8,30,9],[31,1,31,5],[31,6,31,7],[31,8,31,9],[33,10,33,12],[28,1,28,5],[28,6,28,7],[28,8,28,9],[29,1,29,5],[29,6,29,7],[29,8,29,9],[26,1,26,5],[26,6,26,7],[26,8,26,9],[26,10,26,12],[27,1,27,5],[27,6,27,7],[27,8,27,9],[5,1,5,12],[23,0,23,12],[22,0,22,12],[24,1,24,12],[25,1,25,5],[25,6,25,7],[25,8,25,9],[25,10,25,12],[6,1,6,12],[7,0,7,12],[10,1,10,5],[10,6,10,7],[10,8,10,9],[10,10,10,12],[11,6,11,7],[11,8,11,9]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Recommended Thermal Cyclers"]
[null,"The tables below list the thermal cyclers that have been tested by 10x Genomics for all available Xenium In Situ Gene Expression assays. Confirm the right adaptor and thermal cycler is used prior to starting experiment. Failure to use correct adaptor and thermal cycler will result in assay failure. "]
[null,"If an assay option was selected on the Readme sheet, the relevant header will be highlighted.",null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[]
["Xenium v1 ",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(OR(P6=\"Xenium_v1\",P6=\"Xenium_v1_Cell_Seg\"),\"Y\",\"N\")","v":"N"}]
[null,"Xenium In Situ Gene Expression User Guide (CG000582)"]
[null,"Xenium In Situ Gene Expression with Cell Segmentation Staining User Guide (CG000749)"]
[null,"Description",null,null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module ",null,null,null,null,"Bio-Rad",null,1851197]
[false,"Biometra TAdvanced 96 SG with 96-well block (silver, 0.2 mL) and gradient function",null,null,null,null,"Analytik Jena",null,"846-x-070-241 \n(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)"]
[false,"Gradient thermal cycler, XT96 Gradient, with 96-well gradient block and standard lid (silver, 0.2 mL) and gradient function**",null,null,null,null,"VWR",null,"76452-153"]
[false,"MJ Research PTC-200 Thermal Cycler (discontinued)",null,null,null,null,"Marshall Scientific",null,"05434-05"]
[]
["Xenium Protein",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P6=\"Xenium_Protein\",\"Y\",\"N\")","v":"Y"}]
[null,"Xenium In Situ Gene and Protein with Cell Segmentation Staining User Guide (CG000819)"]
[null,"Description",null,null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module",null,null,null,null,"Bio-Rad",null,1851197]
[false,"Biometra TAdvanced 96 S with 96-well block (silver, 0.2 mL)",null,null,null,null,"Analytik Jena",null,"846-x-070-251 \n(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)"]
[false,"Biometra TAdvanced 96 SG with 96-well block (silver, 0.2 mL) and gradient function",null,null,null,null,"Analytik Jena",null,"846-x-070-241 \n(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)"]
[]
["Xenium Prime ",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P6=\"Xenium_Prime\",\"Y\",\"N\")","v":"N"}]
[null,"Xenium Prime In Situ Gene with optional Cell Segmentation Staining User Guide (CG000760)"]
[null,"Description",null,null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module ",null,null,null,null,"Bio-Rad",null,1851197]
[false,"PTC Tempo Deepwell Thermal Cycler ",null,null,null,null,"Bio-Rad",null,12015392]
[false,"Biometra TAdvanced 96 SG with 96-well block\n(silver, 0.2 mL) and gradient function",null,null,null,null,"Analytik Jena ",null,"846-x-070-241 \n(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)"]
[false,"VeritiPro 96-well Thermal Cycler ",null,null,null,null,"Thermo Fisher\nScientific",null,"A48141"]
[false,"Mastercycler X50s - PCR Thermocycler ",null,null,null,null,"Eppendorf",null,6311000010]
[false,"Mastercycler X50a - PCR Thermocycler ",null,null,null,null,"Bio-Rad",null,6313000018]

{"sheet":"Gene Panels","index":4,"n_rows":7,"n_cols":2,"rows_kept":7,"cut":false,"hidden":false,"merged":[[0,1,0,12],[5,1,5,12],[7,1,7,12],[8,1,8,12],[9,1,9,12],[6,1,6,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Gene Panel Selection"]
[]
[null,"Prior to executing the Xenium v1,  Xenium Prime, and Xenium Protein workflows, ensure that a compatible gene panel has been selected. The panels for Xenium v1 and Xenium Prime are not cross-compatible. 10x Genomics provides the option of using pre-designed gene panels. Additionally, the pre-designed panel may be customized by adding genes of interest."]
[null,"For more information on pre-designed panels and requesting custom panel designs, visit the Xenium Panel Designer Support Page on the 10x Genomics Support website. For guidance for selecting genes to include on Xenium add-on panels, see Technical Note CG000643. For information on standalone custom panel design beyond those currently offered by 10x Genomics, see Technical Note CG000683. For all other questions, contact 10x Genomics at customerservice@10xgenomics.com"]

{"sheet":"FFPE Kits & Addtl Reagents","index":5,"n_rows":56,"n_cols":16,"rows_kept":56,"cut":false,"hidden":false,"merged":[[56,0,56,12],[47,3,47,6],[47,7,47,8],[47,9,47,10],[47,11,47,12],[48,1,48,12],[49,1,49,12],[50,11,50,12],[45,11,45,12],[46,3,46,6],[46,11,46,12],[50,7,50,8],[50,9,50,10],[50,3,50,6],[53,1,53,12],[54,1,54,12],[55,1,55,11],[46,7,46,8],[46,9,46,10],[45,1,46,2],[47,1,47,2],[50,1,50,2],[44,1,44,2],[45,3,45,6],[45,7,45,8],[45,9,45,10],[44,9,44,10],[44,11,44,12],[42,1,43,2],[43,3,43,6],[43,7,43,8],[43,9,43,10],[43,11,43,12],[44,3,44,6],[44,7,44,8],[42,9,42,10],[42,11,42,12],[41,1,41,2],[41,3,41,6],[41,7,41,8],[41,9,41,10],[41,11,41,12],[42,3,42,6],[42,7,42,8],[36,11,36,12],[37,3,37,6],[37,11,37,12],[39,3,39,6],[40,3,40,6],[40,7,40,8],[40,9,40,10],[40,11,40,12],[38,3,38,6],[38,7,38,8],[38,9,38,10],[38,11,38,12],[39,7,39,8],[39,9,39,10],[39,11,39,12],[37,1,38,2],[39,1,39,2],[17,11,17,12],[19,9,19,10],[19,11,19,12],[16,1,16,2],[17,1,18,2],[18,3,18,6],[18,7,18,8],[19,1,19,2],[19,3,19,6],[19,7,19,8],[17,3,17,6],[17,7,17,8],[16,9,16,10],[10,1,10,2],[10,3,10,6],[10,7,10,8],[10,9,10,10],[10,11,10,12],[11,3,11,6],[11,11,11,12],[11,1,11,2],[12,1,12,2],[12,3,12,6],[12,7,12,8],[12,9,12,10],[12,11,12,12],[14,11,14,12],[15,1,15,2],[15,3,15,6],[15,7,15,8],[15,9,15,10],[15,11,15,12],[16,3,16,6],[16,7,16,8],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[6,0,6,12],[7,1,7,12],[8,1,8,2],[8,3,8,6],[8,7,8,8],[8,9,8,10],[8,11,8,12],[9,1,9,12],[11,7,11,8],[11,9,11,10],[40,1,40,2],[13,3,13,6],[13,7,13,8],[14,1,14,2],[14,3,14,6],[14,7,14,8],[14,9,14,10],[13,1,13,2],[13,9,13,10],[17,9,17,10],[37,7,37,8],[37,9,37,10],[35,3,35,6],[35,7,35,8],[36,3,36,6],[36,7,36,8],[36,9,36,10],[24,1,24,12],[32,7,32,8],[32,9,32,10],[33,1,33,12],[34,3,34,5],[34,7,34,8],[34,9,34,10],[34,11,34,12],[23,1,23,2],[25,1,25,2],[26,1,26,2],[32,1,32,2],[28,1,28,10],[29,1,29,12],[31,0,31,12],[32,3,32,6],[32,11,32,12],[23,3,23,6],[23,7,23,8],[23,9,23,10],[23,11,23,12],[26,7,26,8],[26,9,26,10],[26,11,26,12],[26,3,26,6],[27,1,27,10],[27,11,27,12],[21,7,21,8],[21,9,21,10],[20,1,20,2],[20,3,20,6],[20,7,20,8],[20,9,20,10],[20,11,20,12],[21,3,21,6],[21,11,21,12],[21,1,21,2],[22,1,22,2],[22,3,22,6],[22,7,22,8],[22,9,22,10],[22,11,22,12],[25,3,25,6],[25,7,25,8],[25,9,25,10],[25,11,25,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Kits, Reagents & Equipment (FFPE)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
["FFPE – Tissue Preparation (CG0000578)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(OR(P4=\"FFPE – GEX\", P4=\"FFPE – PEX\"),\"Y\",\"N\")","v":"N"}]
[null,"Sections placed on the Xenium slide can be stored at room temperature in a desiccator for up to 4 weeks."]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[null,"For FFPE Tissue Sectioning & Section Placement (off-instrument; ~3 h)"]
[false,"Microtome",null,"Epredia HM 355S Automatic Microtome\nOr any standard histology grade microtome",null,null,null,"Fisher Scientific",null,"23-900-672"]
[false,"Microtome Blade",null,"Epredia MX35 Premier Disposable Microtome Blades, Low Profile",null,null,null,"Fisher Scientific",null,3052835]
[false,"Cool-cut \nOptional",null,"Thermo Scientific Cool-Cut\nOptional",null,null,null,"Fisher Scientific",null,"77-112-0"]
[false,"Section transfer system (STS) Optional",null,"Thermo Scientific Section Transfer System (STS)",null,null,null,"Fisher Scientific",null,"771200"]
[false,"Probes",null,"Fisherbrand Fine Precision Probe",null,null,null,"Fisher Scientific",null,"12-000-153"]
[false,"Forceps",null,"Fisherbrand Curved Medium Point General Purpose Forceps",null,null,null,"Fisher Scientific",null,"16-100-110"]
[false,"Blank Slides \nOptional for sectioning practice",null,"Superfrost Plus Microscope Slides",null,null,null,"Fisher Scientific",null,"12-550-15"]
[false,"Water bath",null,"Tissue Floating Bath, Lighted (or equivalent)",null,null,null,"Geyer",null,"194242"]
[null,null,null,"Epredia Digital Round Tissue Section Water bath \nIf using optional Section Transfer System",null,null,null,"Fisher Scientific",null,"A84600061"]
[false,"Section dryer oven\nOptional, but recommended",null,"Epredia High Capacity Section Dryer\nor equivalent. Thermal cycler may also be used for\nsection drying",null,null,null,"Fisher Scientific",null,"A84600051"]
[false,"Brushes",null,"Camel Hair Brushes (or equivalent)",null,null,null,"Ted Pella",null,11859]
[false,"Fan\nFor drying slides",null,"Personal Rechargeable Fan (or equivalent)",null,null,null,"Holmes",null,"085-01-0117"]
[false,"Cutting Mat",null,"WellTech Cutting Mat",null,null,null,"WellTech Precision Lab",null,"-"]
[false,"Wax Trimmer\nOptional",null,"Electronic Microscopy Sciences Paraffin Block Trimmer Wax Trimmer, 115 VAC",null,null,null,"Fisher Scientific",null,"NC0310844"]
[null,"Additional Materials"]
[false,"Razor blades"]
[false,"Ice bucket (4-5 L)"]
[false,"Ultrapure/Milli-Q Water for Water Bath from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]
[]
[]
["FFPE – Deparaffinization & Decrosslinking (CG000578)"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[null,"For FFPE Tissue Sections: Deparaffinization & Decrosslinking (off-instrument; Xenium v1 - ~4.5 h; Xenium Prime ~2.5 h)"]
[false,"Xylene",null,"Xylene, Reagent Grade",null,null,null,"Millipore Sigma",null,214736]
[null,null,null,"Xylene, Histological Grade",null,null,null,"Millipore Sigma",null,534056]
[null,null,null,"Neo-clear Xylene Alternative Substitute\nOnly tested for the Xenium v1 workflow",null,null,null,"Millipore Sigma",null,1098435000]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"Nuclease-free water",null,"Nuclease-free Water (not DEPC-treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4,\nRNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"Urea",null,"Urea Solution, 8M",null,null,null,"Millipore Sigma",null,"51457"]
[false,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution; not 100% Tween diluted to 10%)",null,null,null,"Thermo Fisher Scientific",null,"28320"]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"Forceps",null,"Tweezers, 4\" Wafer Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"Staining jar/dishes",null,"Coplin Jar",null,null,null,"VWR",null,"100500-232"]
[null,null,null,"Staining Dishes",null,null,null,"VWR",null,"25608-906"]
[false,"Section dryer oven",null,"Epredia High Capacity Section Dryer\nor equivalent. Thermal cycler may also be used for section drying",null,null,null,"Fisher Scientific",null,"A84600051"]
[null,"Additional Materials"]
[false,"Water Bath Alternatively, Eppendorf Thermomixer C (5382000023) with SmartBlock -2.0 mL (5362000035) or equivalent may be used"]
[false,"Slide drying rack"]
[false,"Fume hood"]
[false,"Vortex"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]
[null,"Refer to the Xenium Assay and the Xenium Analyzer tabs for reagents & consumables required (applies to both FFPE and FF samples)"]

{"sheet":"FF Kits & Addtl Reagents","index":6,"n_rows":62,"n_cols":16,"rows_kept":62,"cut":false,"hidden":false,"merged":[[44,11,44,12],[45,3,45,6],[45,7,45,8],[47,7,47,8],[47,9,47,10],[45,9,45,10],[45,11,45,12],[46,3,46,6],[46,7,46,8],[46,9,46,10],[46,11,46,12],[47,11,47,12],[41,11,41,12],[42,3,42,6],[42,7,42,8],[42,9,42,10],[42,11,42,12],[43,7,43,8],[43,9,43,10],[43,11,43,12],[43,3,43,6],[41,7,41,8],[41,9,41,10],[38,1,38,2],[38,3,38,6],[38,7,38,8],[38,9,38,10],[38,11,38,12],[39,11,39,12],[39,3,39,6],[40,3,40,6],[40,7,40,8],[40,9,40,10],[40,11,40,12],[39,7,39,8],[39,9,39,10],[24,1,24,2],[25,1,25,2],[26,1,26,2],[28,1,28,2],[29,1,29,2],[31,1,31,2],[8,1,8,2],[11,1,11,2],[12,1,13,2],[18,1,18,2],[20,1,20,2],[21,1,21,2],[27,1,27,12],[28,3,28,6],[28,7,28,8],[28,9,28,10],[28,11,28,12],[9,1,9,12],[10,1,10,11],[11,3,11,6],[11,7,11,8],[11,9,11,10],[11,11,11,12],[12,7,12,8],[12,9,12,10],[12,11,12,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[6,0,6,12],[7,1,7,12],[8,3,8,6],[8,7,8,8],[8,9,8,10],[8,11,8,12],[12,3,12,6],[24,7,24,8],[24,9,24,10],[24,11,24,12],[24,3,24,6],[25,3,25,6],[25,7,25,8],[25,9,25,10],[25,11,25,12],[26,3,26,6],[26,7,26,8],[26,9,26,10],[26,11,26,12],[19,1,19,11],[20,11,20,12],[22,7,22,8],[23,7,23,8],[23,9,23,10],[23,11,23,12],[20,7,20,8],[20,9,20,10],[21,7,21,8],[21,9,21,10],[21,11,21,12],[22,9,22,10],[22,11,22,12],[20,3,20,6],[21,3,21,6],[22,3,22,6],[23,3,23,6],[22,1,22,2],[23,1,23,2],[13,3,13,6],[13,7,13,8],[13,9,13,10],[13,11,13,12],[14,1,14,11],[15,11,15,12],[15,3,15,6],[18,3,18,6],[18,7,18,8],[18,9,18,10],[18,11,18,12],[15,7,15,8],[15,9,15,10],[16,3,16,6],[16,7,16,8],[15,1,17,2],[17,3,17,6],[17,7,17,8],[17,9,17,10],[16,9,16,10],[42,1,43,2],[44,1,45,2],[46,1,46,2],[47,1,47,2],[41,3,41,6],[44,3,44,6],[44,7,44,8],[44,9,44,10],[39,1,41,2],[34,0,34,12],[35,1,35,2],[35,7,35,8],[35,9,35,10],[35,11,35,12],[35,3,35,6],[36,1,36,12],[37,1,37,2],[37,3,37,6],[37,7,37,8],[37,9,37,10],[37,11,37,12],[29,3,29,6],[29,7,29,8],[29,9,29,10],[29,11,29,12],[31,7,31,8],[31,9,31,10],[31,11,31,12],[31,3,31,6],[32,1,32,12],[59,1,59,12],[60,1,60,12],[61,1,61,12],[56,3,56,6],[57,3,57,6],[57,7,57,8],[57,9,57,10],[57,11,57,12],[58,1,58,10],[58,11,58,12],[57,1,57,2],[56,7,56,8],[56,9,56,10],[56,11,56,12],[56,1,56,2],[51,1,51,2],[53,3,53,6],[53,7,53,8],[53,9,53,10],[53,11,53,12],[54,3,54,6],[54,11,54,12],[55,3,55,6],[55,7,55,8],[55,9,55,10],[55,11,55,12],[54,1,54,2],[54,7,54,8],[54,9,54,10],[51,3,51,6],[51,7,51,8],[51,9,51,10],[51,11,51,12],[52,1,52,10],[52,11,52,12],[53,1,53,2],[55,1,55,2],[49,9,49,10],[49,11,49,12],[47,3,47,6],[48,3,48,6],[48,7,48,8],[48,9,48,10],[48,11,48,12],[49,7,49,8],[50,1,50,12],[49,3,49,6],[48,1,48,2],[49,1,49,2]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Kits, Reagents & Equipment (Fresh Frozen)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
["Fresh Frozen – Tissue Preparation (CG000579)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P4=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]
[null,"Sections placed on the Xenium slide can be stored at -80ºC for up to 4 weeks."]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[null,"For FF Tissue Sectioning & Section Placement (off-instrument; ~3 h)"]
[null,"Tissue Freezing"]
[false,"Isopentane",null,"Isopentane (2-Methylbutane)",null,null,null,"Millipore Sigma",null,270342]
[false,"Forceps",null,"Specimen Forceps, Straight, 203 mm (8\")",null,null,null,"VWR",null,"82027-436"]
[null,null,null,"Specimen Forceps, Straight, 152 mm (6\")",null,null,null,"VWR",null,"82027-438"]
[null,"Frozen Tissue Embedding"]
[false,"Embedding Compound",null,"TissueTek O.C.T. Compound",null,null,null,"VWR",null,"25608-930"]
[null,null,null,"Fisher Healthcare Tissue-Plus O.C.T. Compound",null,null,null,"Fisher Scientific",null,"23-730-571"]
[null,null,null,"Tissue Freezing Media",null,null,null,"Leica",null,14020108926]
[false,"Embedding Molds",null,"Epredia Peel-A-Way Disposable Embedding Molds",null,null,null,"Fisher Scientific",null,"12-20"]
[null,"Frozen Tissue Sectioning"]
[false,"Blank Slides Optional, for sectioning practice",null,"Superfrost Plus Microscope Slides",null,null,null,"Fisher Scientific",null,"12-550-15"]
[false,"Cryostat",null,"CryoStar NX70 Cryostat",null,null,null,"Fisher Scientific",null,957020]
[false,"Brushes",null,"Flat cryostat brush, 10 mm (or equivalent)",null,null,null,"Fisher Scientific",null,"14-071-00"]
[false,"Specimen Chuck",null,"Thermo Scientific CryoStar NX70 Specimen Chuck",null,null,null,"Fisher Scientific",null,"14-071-413"]
[false,"Microtome Blade",null,"MX35 Ultra Microtome Blade, Low Profile",null,null,null,"Fisher Scientific",null,3051835]
[false,"Slide Mailer",null,"Simport Scientific LockMailer Tamper Evident Slide\nMailer",null,null,null,"Fisher Scientific",null,"22-038-399"]
[false,"Anti-Roll Plate Optional",null,"Glass Anti-Roll Plate",null,null,null,"Fisher Scientific",null,"A78930200"]
[null,"Additional Materials"]
[false,"Dry ice"]
[false,"Razor blades"]
[false,"Ice bucket"]
[false,"Aluminum Foil"]
[null,"This list may not include some standard laboratory equipment."]
[]
["Fresh Frozen – Fixation and Permeabilization (CG000579)"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[null,"For FF Tissue Sections: Fixation & Permeabilization (off-instrument; ~2.5 h)"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"Nuclease-free Water",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[false,"Formaldehyde or Paraformaldehyde",null,"Formaldehyde (37% by Weight/Molecular Biology)",null,null,null,"Thermo Fisher Scientific",null,"BP531-500"]
[null,null,null,"Formaldehyde solution",null,null,null,"Millipore Sigma",null,"252549/ F8775/47608"]
[null,null,null,"Paraformaldehyde 16% Aqueous Solution, EM Grade",null,null,null,"Electron Microscopy\nSciences",null,"15710"]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution; not 100% Tween diluted to 10%)",null,null,null,"Thermo Fisher Scientific",null,"28320"]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"Methanol",null,"Methanol, for HPLC",null,null,null,"Millipore Sigma",null,"34860"]
[false,"SDS",null,"Sodium dodecyl sulfate solution (for molecular biology,\n10% in H2O)",null,null,null,"Millipore Sigma",null,"71736"]
[false,"Forceps",null,"Tweezers, 4\" Wafer Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"Slide Mailers",null,"Sim port Scientific LockMailer Tamper Evident Slide Mailer",null,null,null,"Fisher Scientific",null,"22-038-399"]
[null,"Additional Materials"]
[false,"Dry ice"]
[false,"10x Genomics has tested only the listed thermal cyclers. Currently no alternate recommendations are available. Use one of the listed thermal cyclers based on preference and availability.\nRefer to the relevant Demonstrated Protocols and User Guides for the recommended thermal cyclers."]
[false,"Slide drying rack"]
[false,"Serological pipettes"]
[false,"Fume hood"]
[false,"Vortex"]
[false,"Ice bucket"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]
[]
[null,"Refer to the Xenium Assay and the Xenium Analyzer tabs for reagents & consumables required (applies to both FFPE and FF samples)"]

{"sheet":"Assay Steps & Timing","index":7,"n_rows":76,"n_cols":11,"rows_kept":76,"cut":false,"hidden":true,"merged":[[57,6,57,7],[57,8,57,9],[54,10,54,12],[57,10,57,12],[52,2,52,5],[52,6,52,7],[52,8,52,9],[52,10,52,12],[53,1,53,12],[54,8,54,9],[56,1,56,12],[76,1,76,12],[77,1,77,12],[66,2,66,5],[67,1,67,12],[68,2,68,5],[68,10,68,12],[69,6,69,7],[69,10,69,12],[70,1,70,12],[66,6,66,7],[66,8,66,9],[71,2,71,5],[73,2,73,5],[74,2,74,5],[71,10,71,12],[72,1,72,12],[73,10,73,12],[74,6,74,7],[74,10,74,12],[75,1,75,9],[62,1,62,12],[61,2,61,5],[63,2,63,5],[63,8,63,9],[63,10,63,12],[64,2,64,5],[65,2,65,5],[66,10,66,12],[69,2,69,5],[38,2,38,5],[39,2,39,5],[39,6,39,7],[39,10,39,12],[40,1,40,12],[41,10,41,12],[42,1,42,12],[41,2,41,5],[43,2,43,5],[43,10,43,12],[44,10,44,12],[61,8,61,9],[61,10,61,12],[58,1,58,12],[59,2,59,5],[59,8,59,9],[59,10,59,12],[60,1,60,12],[61,6,61,7],[44,2,44,5],[44,6,44,7],[45,1,45,9],[48,1,48,5],[48,6,48,7],[48,8,48,9],[48,10,48,12],[49,1,49,12],[50,2,50,5],[50,8,50,9],[50,10,50,12],[51,2,51,5],[54,2,54,5],[55,2,55,5],[57,2,57,5],[13,2,13,5],[15,2,15,5],[17,2,17,5],[18,2,18,5],[18,6,18,7],[10,2,10,5],[11,2,11,5],[11,6,11,7],[11,8,11,9],[16,1,16,12],[17,8,17,9],[17,10,17,12],[18,8,18,9],[18,10,18,12],[11,10,11,12],[12,1,12,12],[14,1,14,12],[36,2,36,5],[36,6,36,7],[36,8,36,9],[36,10,36,12],[37,1,37,12],[38,10,38,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[7,0,7,13],[8,1,8,5],[8,6,8,7],[8,8,8,9],[8,10,8,12],[9,1,9,12],[10,8,10,9],[10,10,10,12],[13,8,13,9],[13,10,13,12],[15,8,15,9],[15,10,15,12],[32,1,32,12],[31,2,31,5],[33,2,33,5],[33,8,33,9],[33,10,33,12],[34,1,34,12],[35,8,35,9],[35,10,35,12],[35,2,35,5],[26,1,26,12],[27,2,27,5],[31,8,31,9],[31,10,31,12],[28,2,28,5],[29,2,29,5],[29,6,29,7],[29,8,29,9],[29,10,29,12],[30,1,30,12],[27,8,27,9],[27,10,27,12],[19,1,19,12],[20,2,20,5],[20,10,20,12],[21,6,21,7],[21,10,21,12],[22,1,22,9],[21,2,21,5],[25,1,25,5],[25,6,25,7],[25,8,25,9],[25,10,25,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Xenium Assay Key Protocol Steps and Timing (off-instrument; for both FFPE & FF samples)"]
[null,"If a workflow option was selected on the Readme sheet, the relevant information will be highlighted."]
[null,"When pairing Xenium v1 or Xenium Prime workflows with Cell Segmentation Staining, process two slides per run. The Xenium Stain Enhancer (PN-2000992) cannot be stored once resuspended. Therefore, the 10x reagents for Xenium v1 and Xenium Prime Cell Segmentation workflows can only be used for one round, regardless of whether one or two slides are processed in a single run."]
[]
["Xenium In Situ Gene Expression (off-instrument; ~2 d; CG000584)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 2: Post Hybridization Wash"]
[null,2.1,"Post Hybridization Wash",null,null,null,"35 min",null,"-"]
[null,"Step 3: Ligation"]
[null,3.1,"Ligation",null,null,null,"~2 h",null,"-"]
[null,"Step 4: Amplification"]
[null,4.0999999999999996,"Amplification",null,null,null,"~ 2 h",null,"-"]
[null,4.2,"Post-Amplification Wash",null,null,null,"15 min",null,"-"]
[null,"Step 5: Autofluorescence Quenching"]
[null,5.0999999999999996,"Autofluorescence Quenching",null,null,null,"30 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,5.2,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]
[]
["Xenium In Situ Gene Expression with Cell Segmentation Staining (off-instrument; ~3 d; CG000749)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Custom Probe Preparation (optional)",null,null,null,"10 min",null,"-"]
[null,1.3,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 2: Post Hybridization Wash"]
[null,2.1,"Post Hybridization Wash",null,null,null,"35 min",null,"-"]
[null,"Step 3: Ligation"]
[null,3.1,"Ligation",null,null,null,"~2 h",null,"-"]
[null,"Step 4: Amplification"]
[null,4.0999999999999996,"Amplification",null,null,null,"~ 2 h",null,"-"]
[null,4.2,"Post-Amplification Wash",null,null,null,"15 min",null,"-"]
[null,"Step 5: Cell Segmentation Staining"]
[null,5.0999999999999996,"Buffer Preparation",null,null,null,"20 min",null,"-"]
[null,5.2,"Block and Stain",null,null,null,"~1 h + 16-24 h (overnight)",null,"-"]
[null,"Day 3"]
[null,5.3,"Stain Enhancement",null,null,null,"~1 h",null,"-"]
[null,"Step 6: Autofluorescence Quenching"]
[null,6.1,"Autofluorescence Quenching",null,null,null,"30 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,6.2,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]
[]
["Xenium Prime In Situ Gene Expression with optional Cell Segmentation Staining (off-instrument; ~3 d; CG000760)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Priming Hybridization",null,null,null,"~1.8 h",null,"-"]
[null,1.3,"Post Priming Hybridization Wash",null,null,null,"70 min",null,"-"]
[null,"Step 2: Post Hybridization Wash"]
[null,2.1,"RNase Treatment",null,null,null,"30 min",null,"-"]
[null,2.2000000000000002,"Polishing",null,null,null,"70 min",null,"-"]
[null,"Step 3: Probe Hybridization"]
[null,3.1,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 4: Post Hybridization Wash"]
[null,4.0999999999999996,"Post Hybridization Wash",null,null,null,"35 min",null,"-"]
[null,"Step 5: Ligation"]
[null,5.0999999999999996,"Ligation",null,null,null,"40 min",null,"-"]
[null,"Step 6: Amplification"]
[null,6.1,"Amplification Enhancement",null,null,null,"~2 h",null,"-"]
[null,6.2,"Post-Amplification Enhancement Wash",null,null,null,"5 min",null,"-"]
[null,6.3,"Amplification",null,null,null,"~1.6 h",null,"-"]
[null,6.4,"Post-Amplification Wash",null,null,null,"15 min",null,"-"]
[null,"OPTIONAL: Step 7: Cell Segmentation Staining"]
[null,7.1,"Buffer Preparation",null,null,null,"20 min",null,"-"]
[null,7.2,"Block and Stain",null,null,null,"~1 h + 16-24 h (overnight)",null,"-"]
[null,"Day 3"]
[null,7.3,"Stain Enhancement",null,null,null,"~1 h",null,"-"]
[null,"Step 8: Autofluorescence Quenching"]
[null,8.1,"Autofluorescence Quenching",null,null,null,"45 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,8.1999999999999993,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]

{"sheet":"Pre-run QC","index":8,"n_rows":98,"n_cols":16,"rows_kept":98,"cut":false,"hidden":false,"merged":[[28,1,28,12],[29,1,29,2],[29,3,29,6],[29,7,29,8],[29,9,29,10],[29,11,29,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[8,3,8,5],[8,7,8,8],[8,9,8,10],[8,11,8,12],[9,3,9,6],[11,1,12,2],[12,3,12,6],[12,7,12,8],[12,9,12,10],[12,11,12,12],[11,3,11,6],[11,7,11,8],[32,1,32,2],[32,3,32,6],[32,7,32,8],[32,9,32,10],[32,11,32,12],[30,1,30,2],[30,3,30,6],[30,7,30,8],[30,9,30,10],[30,11,30,12],[31,1,31,2],[31,3,31,6],[31,7,31,8],[31,9,31,10],[31,11,31,12],[36,11,36,12],[37,3,37,6],[37,7,37,8],[34,1,34,12],[35,1,35,2],[35,3,35,6],[35,7,35,8],[35,9,35,10],[35,11,35,12],[38,3,38,6],[38,7,38,8],[39,1,39,2],[39,3,39,6],[39,7,39,8],[39,9,39,10],[36,3,36,6],[36,7,36,8],[36,9,36,10],[40,1,40,2],[40,3,40,6],[40,7,40,8],[40,9,40,10],[41,1,42,2],[41,3,41,6],[41,7,41,8],[41,9,41,10],[42,3,42,6],[42,7,42,8],[42,9,42,10],[42,11,42,12],[43,1,44,2],[43,3,43,6],[43,7,43,8],[43,9,43,10],[43,11,43,12],[44,3,44,6],[44,7,44,8],[44,9,44,10],[44,11,44,12],[45,3,45,5],[45,7,45,8],[45,9,45,10],[45,11,45,12],[46,3,46,6],[46,7,46,8],[46,9,46,10],[46,11,46,12],[47,1,47,2],[47,3,47,6],[47,7,47,8],[47,9,47,10],[47,11,47,12],[48,1,48,2],[48,3,48,6],[48,7,48,8],[48,9,48,10],[48,11,48,12],[52,11,52,12],[53,1,53,2],[53,3,53,6],[53,7,53,8],[53,9,53,10],[53,11,53,12],[49,3,49,6],[49,7,49,8],[49,9,49,10],[49,11,49,12],[50,3,50,6],[50,7,50,8],[50,9,50,10],[50,11,50,12],[56,1,56,12],[57,1,57,12],[36,1,38,2],[6,1,6,12],[7,1,7,2],[7,3,7,6],[7,7,7,8],[7,9,7,10],[7,11,7,12],[22,1,22,2],[54,1,54,2],[54,3,54,6],[54,7,54,8],[54,9,54,10],[54,11,54,12],[55,1,55,10],[55,11,55,12],[51,1,51,12],[52,1,52,10],[9,7,9,8],[10,3,10,6],[10,7,10,8],[10,9,10,10],[10,11,10,12],[11,9,11,10],[11,11,11,12],[14,1,14,2],[14,3,14,6],[14,7,14,8],[14,9,14,10],[14,11,14,12],[13,1,13,2],[13,3,13,6],[13,7,13,8],[13,9,13,10],[13,11,13,12],[17,1,17,2],[17,3,17,6],[17,7,17,8],[17,9,17,10],[17,11,17,12],[19,1,19,12],[15,1,16,2],[15,3,15,6],[15,7,15,8],[15,9,15,10],[15,11,15,12],[16,3,16,6],[16,7,16,8],[16,9,16,10],[16,11,16,12],[21,3,21,6],[21,7,21,8],[21,9,21,10],[21,11,21,12],[22,3,22,6],[22,7,22,8],[22,9,22,10],[22,11,22,12],[20,1,20,2],[20,3,20,6],[20,7,20,8],[20,9,20,10],[20,11,20,12],[21,1,21,2],[25,7,25,8],[25,9,25,10],[25,11,25,12],[23,1,25,2],[23,3,23,6],[23,7,23,8],[23,9,23,10],[23,11,23,12],[24,3,24,6],[24,7,24,8],[24,9,24,10],[24,11,24,12],[25,3,25,6],[27,1,27,2],[27,3,27,6],[27,7,27,8],[27,9,27,10],[27,11,27,12],[26,1,26,2],[26,3,26,6],[26,7,26,8],[26,9,26,10],[26,11,26,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["DAPI and H&E Quality Assessment - Reagents & Consumables (not supplied by 10x Genomics)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available. Refer to the manufacturer's website for regional part numbers."]
[]
[null,"For Deparaffinization (FFPE only, prior to DAPI and H&E staining)"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Xylene",null,"Xylene, Reagent Grade",null,null,null,"Millipore Sigma",null,214736]
[null,null,null,"Xylene, Histological Grade",null,null,null,"Millipore Sigma",null,534056]
[null,null,null,"Neo-clear Xylene Alternative Substitute\nOnly tested for the Xenium v1 workflow",null,null,null,"Millipore Sigma",null,1098435000]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"Nuclease-free water",null,"Nuclease-free Water (not DEPC-treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[false,"Forceps",null,"Tweezers, 4\" Wafer Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"Staining jar/dishes",null,"Coplin Jar",null,null,null,"VWR",null,"100500-232"]
[null,null,null,"Staining Dishes",null,null,null,"VWR",null,"25608-906"]
[false,"Section dryer oven",null,"Epredia High Capacity Section Dryer\nor equivalent. Thermal cycler may also be used for section drying",null,null,null,"Fisher Scientific",null,"A84600051"]
[]
[null,"For Fixation (FFPE only, prior to DAPI and H&E staining)",null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P4=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"Nuclease-free Water",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[false,"Formaldehyde or Paraformaldehyde",null,"Formaldehyde (37% by Weight/Molecular Biology)",null,null,null,"Thermo Fisher Scientific",null,"BP531-500"]
[null,null,null,"Formaldehyde solution",null,null,null,"Millipore Sigma",null,"252549/ F8775/47608"]
[null,null,null,"Paraformaldehyde 16% Aqueous Solution, EM Grade",null,null,null,"Electron Microscopy Sciences",null,"15710"]
[false,"Forceps",null,"Tweezers, 4\" Wafer Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"Slide Mailers",null,"Sim port Scientific LockMailer Tamper Evident Slide Mailer",null,null,null,"Fisher Scientific",null,"22-038-399"]
[null,"For DAPI Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"DAPI",null,"Use any preferred DAPI",null,null,null,"-",null,"-"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"Slide Mailers",null,"Sim port Scientific LockMailer Tamper Evident Slide\nMailer",null,null,null,"Fisher Scientific",null,"22-038-399"]
[]
[null,"For H&E Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Hematoxylin",null,"Hematoxylin Solution, Mayer's",null,null,null,"Sigma Aldrich",null,"MHS16"]
[null,null,null,"EMS Mayer's Hematoxylin",null,null,null,"Electron Microscopy Sciences",null,"26043-05"]
[null,null,null,"Hematoxylin, Mayer's, Classical",null,null,null,"Ethos Biosciences",null,"7021"]
[false,"Eosin",null,"Eosin Y Solution, Alcoholic",null,null,null,"Leica",null,3801615]
[false,"Bluing Reagent",null,"Bluing Solution",null,null,null,"Dako",null,"CS702"]
[false,"Mounting Media",null,"Surgipath SUB-X Mounting Media Discontd.",null,null,null,"Leica",null,3801741]
[null,null,null,"Epredia Cytoseal Mountant (Cytoseal or equivalent mounting media can be used)",null,null,null,"Fisher Scientific",null,"22-050-262"]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure  (Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"Xylene",null,"Xylene, Reagent Grade",null,null,null,"Millipore Sigma",null,214736]
[null,null,null,"Xylene, Histological Grade",null,null,null,"Millipore Sigma",null,534056]
[false,"Forceps",null,"Tweezers, 4' Water Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"Filter Paper",null,"Fisherbrand Qualitative Grade Plain Filter Paper Circles",null,null,null,"Fisher Scientific",null,"09-795-H"]
[false,"Coverslips",null,"Fisherbrand Cover Glasses: Rectangles Discontinued",null,null,null,"Fisher Scientific",null,"12-544-EP"]
[null,null,null,"Cover Glasses, Rectangles",null,null,null,"VWR",null,"16004-322"]
[null,"Additional Materials"]
[false,"Vortex"]
[false,"Staining jar/dishes"]
[false,"Wide-bore pipette tips"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]
[]
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[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P95=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]

{"sheet":"Xenium v1 Workflows","index":9,"n_rows":85,"n_cols":12,"rows_kept":85,"cut":false,"hidden":false,"merged":[[73,9,73,10],[73,11,73,12],[74,9,74,10],[74,11,74,12],[67,3,67,6],[68,3,68,5],[68,6,70,6],[68,7,70,8],[68,11,68,12],[69,3,69,5],[70,11,70,12],[70,3,70,5],[71,3,71,5],[71,6,74,6],[71,7,74,8],[72,3,72,5],[73,3,73,5],[74,3,74,5],[67,7,67,8],[67,9,67,10],[67,11,67,12],[69,9,69,10],[69,11,69,12],[68,9,68,10],[70,9,70,10],[71,9,71,10],[71,11,71,12],[61,3,61,6],[61,7,61,8],[61,9,61,10],[61,11,61,12],[62,3,62,6],[62,7,62,8],[66,3,66,6],[66,7,66,8],[66,9,66,10],[66,11,66,12],[64,3,64,6],[65,3,65,6],[65,7,65,8],[65,9,65,10],[65,11,65,12],[63,3,63,6],[63,7,63,8],[63,9,63,10],[63,11,63,12],[64,7,64,8],[64,9,64,10],[82,1,82,10],[82,11,82,12],[83,1,83,10],[83,11,83,12],[84,1,84,12],[80,3,80,6],[80,7,80,8],[80,9,80,10],[80,11,80,12],[81,7,81,8],[81,9,81,10],[81,11,81,12],[75,3,75,6],[75,7,75,8],[75,9,75,10],[75,11,75,12],[76,7,76,8],[76,9,76,10],[76,11,76,12],[76,3,76,6],[77,1,77,12],[56,1,56,2],[57,1,58,2],[59,1,60,2],[61,1,61,2],[62,1,63,2],[76,1,76,2],[79,1,79,2],[80,1,80,2],[81,1,81,2],[64,1,65,2],[66,1,66,2],[67,1,67,2],[68,1,68,2],[70,1,70,2],[71,1,74,2],[75,1,75,2],[78,1,78,12],[79,3,79,6],[79,7,79,8],[79,9,79,10],[79,11,79,12],[81,3,81,6],[62,9,62,10],[62,11,62,12],[57,3,57,6],[57,7,57,8],[57,9,57,10],[57,11,57,12],[58,3,58,6],[58,7,58,8],[60,7,60,8],[60,9,60,10],[58,9,58,10],[58,11,58,12],[59,3,59,6],[59,7,59,8],[59,9,59,10],[59,11,59,12],[60,11,60,12],[60,3,60,6],[45,1,45,9],[47,0,47,12],[48,1,48,12],[49,1,49,12],[50,0,50,12],[51,1,51,12],[43,10,43,12],[44,10,44,12],[38,2,38,5],[39,2,39,5],[39,6,39,7],[39,10,39,12],[40,1,40,12],[41,10,41,12],[42,1,42,12],[41,2,41,5],[43,2,43,5],[44,2,44,5],[44,6,44,7],[26,1,26,12],[27,2,27,5],[31,8,31,9],[31,10,31,12],[28,2,28,5],[29,2,29,5],[29,6,29,7],[29,8,29,9],[29,10,29,12],[30,1,30,12],[31,2,31,5],[27,8,27,9],[27,10,27,12],[20,2,20,5],[20,10,20,12],[21,6,21,7],[21,10,21,12],[22,1,22,9],[21,2,21,5],[25,1,25,5],[25,6,25,7],[25,8,25,9],[25,10,25,12],[64,11,64,12],[54,7,54,8],[54,9,54,10],[52,1,52,2],[53,3,53,6],[53,7,53,8],[53,9,53,10],[53,11,53,12],[54,3,54,6],[54,11,54,12],[52,3,52,6],[52,7,52,8],[52,9,52,10],[52,11,52,12],[55,3,55,6],[55,7,55,8],[55,9,55,10],[55,11,55,12],[53,1,54,2],[55,1,55,2],[56,7,56,8],[56,9,56,10],[56,11,56,12],[56,3,56,6],[37,1,37,12],[38,10,38,12],[18,8,18,9],[18,10,18,12],[13,2,13,5],[15,2,15,5],[17,2,17,5],[18,2,18,5],[18,6,18,7],[17,8,17,9],[17,10,17,12],[32,1,32,12],[33,2,33,5],[33,8,33,9],[33,10,33,12],[34,1,34,12],[35,8,35,9],[35,10,35,12],[35,2,35,5],[36,2,36,5],[36,6,36,7],[36,8,36,9],[36,10,36,12],[19,1,19,12],[10,2,10,5],[11,2,11,5],[11,6,11,7],[11,8,11,9],[11,10,11,12],[12,1,12,12],[14,1,14,12],[16,1,16,12],[9,1,9,12],[10,8,10,9],[10,10,10,12],[13,8,13,9],[13,10,13,12],[15,8,15,9],[15,10,15,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[7,0,7,13],[8,1,8,5],[8,6,8,7],[8,8,8,9],[8,10,8,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Xenium v1 Assay Key Protocol Steps and Timing (off-instrument; for both FFPE & FF samples)"]
[null,"If an assay option was selected on the Readme sheet, the relevant information will be highlighted."]
[null,"When performing cell segmentation staining, process two slides per run. The Xenium Stain Enhancer (PN-2000992) cannot be stored once resuspended. It can only be used for one run, regardless of whether one or two slides are processed in a single run."]
[]
["Xenium In Situ Gene Expression (off-instrument; ~2 d; CG000584)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 2: Post Hybridization Wash"]
[null,2.1,"Post Hybridization Wash",null,null,null,"35 min",null,"-"]
[null,"Step 3: Ligation"]
[null,3.1,"Ligation",null,null,null,"~2 h",null,"-"]
[null,"Step 4: Amplification"]
[null,4.0999999999999996,"Amplification",null,null,null,"~ 2 h",null,"-"]
[null,4.2,"Post-Amplification Wash",null,null,null,"15 min",null,"-"]
[null,"Step 5: Autofluorescence Quenching"]
[null,5.0999999999999996,"Autofluorescence Quenching",null,null,null,"30 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,5.2,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]
[]
["Xenium In Situ Gene Expression with Cell Segmentation Staining (off-instrument; ~3 d; CG000749)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Custom Probe Preparation (optional)",null,null,null,"10 min",null,"-"]
[null,1.3,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 2: Post Hybridization Wash"]
[null,2.1,"Post Hybridization Wash",null,null,null,"35 min",null,"-"]
[null,"Step 3: Ligation"]
[null,3.1,"Ligation",null,null,null,"~2 h",null,"-"]
[null,"Step 4: Amplification"]
[null,4.0999999999999996,"Amplification",null,null,null,"~ 2 h",null,"-"]
[null,4.2,"Post-Amplification Wash",null,null,null,"15 min",null,"-"]
[null,"Step 5: Cell Segmentation Staining"]
[null,5.0999999999999996,"Buffer Preparation",null,null,null,"20 min",null,"-"]
[null,5.2,"Block and Stain",null,null,null,"~1 h + 16-24 h (overnight)",null,"-"]
[null,"Day 3"]
[null,5.3,"Stain Enhancement",null,null,null,"~1 h",null,"-"]
[null,"Step 6: Autofluorescence Quenching"]
[null,6.1,"Autofluorescence Quenching",null,null,null,"30 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,6.2,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]
[]
["Xenium v1 Assay Kits, Reagents & Equipment"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
["Xenium v1 Assay Workflow (CG000582 or CG000749)"]
[null,"Xenium In Situ Gene Expression ± Cell Segmentation Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Nuclease-free Water\n(select one based on availability",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[null,null,null,"Nuclease-free Milli-Q water (Biopak® Polisher)\n",null,null,null,"Millipore Sigma",null,"CDUFBI0A1"]
[false,"TE Buffer",null,"TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0",null,null,null,"Fisher Scientific",null,"BP24731"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"Glycerol",null,"Glycerol, 99.5 % Molecular Biology, DNAse, RNAse, Protease free (optional, if storing slides long-term)",null,null,null,"Acros Organics",null,"327255000"]
[false,"1.5 ml Tubes",null,"DNA LoBindTubes, 1.5 ml",null,null,null,"Eppendorf",null,22431021]
[null,null,null,"Low DNA Binding Tubes, 1.5 ml",null,null,null,"Sarstedt",null,"72.706.700"]
[false,"2.0 ml Tubes",null,"DNA LoBindTubes, 2.0 ml",null,null,null,"Eppendorf",null,22431048]
[null,null,null,"Low DNA Binding Tubes, 2.0 ml",null,null,null,"Sarstedt",null,"72.695.700"]
[false,"15 ml Tubes",null,"15 ml PP Centrifuge Tubes",null,null,null,"Corning",null,"730791"]
[false,"50 ml Tubes",null,"Self-Standing Polypropylene Centrifuge Tubes, sterile",null,null,null,"Corning",null,"430921"]
[false,"Pipette Tips",null,"Tips LTS 200UL Filter RT-L200 FLR",null,null,"(or equivalent)","Rainin",null,30389240]
[null,null,null,"Tips LTS 1ML Filter RT-L1000 FLR",null,null,null,null,null,30389213]
[null,null,null,"Tips LTS 20UL Filter RT-L20 FLR",null,null,null,null,null,30389226]
[false,"Pipettes",null,"Pipet-Lite LTS Pipette L-20XLS+",null,null,"(or equivalent)","Rainin",null,17014392]
[null,null,null,"Pipet-Lite LTS Pipette L-100XLS+",null,null,null,null,null,17014384]
[null,null,null,"Pipet-Lite LTS Pipette L-200XLS+",null,null,null,null,null,17014391]
[null,null,null,"Pipet-Lite LTS Pipette L-1000XLS+",null,null,null,null,null,17014382]
[false,"Blank Slides",null,"Superfrost Plus Slides (optional, if practicing Xenium Cassette Insert assembly)",null,null,null,"Fisherbrand",null,"12-550-15"]
[false,"Forceps",null,"Fisherbrand Curved Medium Point General Purpose Forceps (or equivalent)",null,null,null,"Fisher Scientific",null,"16-100-110"]
[null,"Additional Materials"]
[false,"Water Bath Alternatively, Eppendorf Thermomixer C (5382000023) with SmartBlock -2.0 mL (5362000035) or equivalent may be used"]
[false,"Mini centrifuge"]
[false,"Vortex"]
[false,"Ice Bucket"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[false,"PCR Sealing Film (optional, for storing slides long-term for Xenium Cassette Insert Practice)"]
[null,"This list may not include some standard laboratory equipment."]

{"sheet":"Xenium Protein Workflow","index":10,"n_rows":74,"n_cols":12,"rows_kept":74,"cut":false,"hidden":false,"merged":[[65,1,65,2],[65,11,65,12],[74,1,74,12],[75,1,75,12],[70,3,70,6],[70,7,70,8],[71,1,71,10],[71,11,71,12],[72,1,72,10],[72,11,72,12],[73,1,73,12],[70,9,70,10],[70,11,70,12],[68,1,68,2],[69,1,69,2],[69,3,69,6],[69,7,69,8],[69,9,69,10],[69,11,69,12],[70,1,70,2],[65,3,65,6],[66,1,66,12],[67,1,67,12],[68,3,68,6],[57,9,57,10],[57,11,57,12],[58,3,58,5],[62,3,62,5],[63,3,63,5],[59,1,59,2],[59,3,59,5],[60,1,63,2],[60,3,60,5],[60,6,63,6],[60,7,63,8],[61,3,61,5],[58,9,58,10],[58,11,58,12],[64,9,64,10],[64,11,64,12],[56,1,56,2],[56,3,56,6],[56,7,56,8],[56,9,56,10],[56,11,56,12],[68,7,68,8],[68,9,68,10],[68,11,68,12],[59,9,59,10],[59,11,59,12],[60,9,60,10],[60,11,60,12],[62,9,62,10],[62,11,62,12],[63,9,63,10],[63,11,63,12],[65,7,65,8],[65,9,65,10],[57,1,57,2],[57,3,57,5],[57,6,59,6],[57,7,59,8],[53,1,54,2],[53,3,53,6],[54,3,54,6],[55,1,55,2],[55,3,55,6],[54,7,54,8],[55,7,55,8],[51,7,51,8],[64,1,64,2],[64,3,64,6],[64,7,64,8],[55,9,55,10],[55,11,55,12],[52,7,52,8],[52,9,52,10],[53,7,53,8],[53,9,53,10],[53,11,53,12],[54,9,54,10],[54,11,54,12],[51,9,51,10],[50,1,50,2],[50,3,50,6],[50,7,50,8],[50,9,50,10],[50,11,50,12],[51,1,52,2],[51,11,51,12],[52,11,52,12],[51,3,51,6],[52,3,52,6],[45,3,45,6],[46,3,46,6],[47,3,47,6],[48,3,48,6],[49,3,49,6],[42,3,42,6],[42,7,42,8],[43,1,44,2],[43,3,43,6],[43,7,43,8],[44,7,44,8],[45,1,49,2],[42,9,42,10],[42,11,42,12],[43,9,43,10],[43,11,43,12],[44,9,44,10],[44,11,44,12],[39,1,39,2],[40,1,40,2],[40,3,40,6],[40,7,40,8],[40,9,40,10],[40,11,40,12],[41,1,42,2],[44,3,44,6],[31,0,31,12],[36,9,36,10],[36,11,36,12],[37,7,37,8],[37,9,37,10],[41,9,41,10],[41,11,41,12],[41,3,41,6],[41,7,41,8],[39,7,39,8],[39,9,39,10],[37,1,38,2],[38,3,38,6],[38,7,38,8],[38,9,38,10],[38,11,38,12],[39,3,39,6],[39,11,39,12],[22,6,22,7],[24,1,24,12],[25,10,25,12],[26,1,26,12],[27,10,27,12],[28,6,28,7],[28,10,28,12],[22,2,22,5],[29,1,29,9],[13,1,13,12],[14,2,14,5],[14,8,14,9],[14,10,14,12],[15,1,15,12],[17,1,17,12],[36,3,36,6],[37,3,37,6],[32,1,32,12],[33,1,33,12],[34,0,34,12],[35,1,35,12],[36,1,36,2],[36,7,36,8],[37,11,37,12],[23,6,23,7],[23,10,23,12],[23,2,23,5],[25,2,25,5],[27,2,27,5],[28,2,28,5],[20,1,20,12],[21,2,21,5],[21,10,21,12],[16,8,16,9],[16,10,16,12],[18,8,18,9],[18,10,18,12],[19,10,19,12],[16,2,16,5],[18,2,18,5],[19,2,19,5],[19,6,19,7],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[8,10,8,12],[12,6,12,7],[12,8,12,9],[8,1,8,5],[9,1,9,12],[10,2,10,5],[10,8,10,9],[10,10,10,12],[11,2,11,5],[12,10,12,12],[12,2,12,5],[8,6,8,7],[8,8,8,9]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene and Protein Expression"]
[]
["Xenium In Situ Gene and Protein Expression Assay Key Protocol Steps and Timing (off-instrument; for FFPE samples)"]
[null,"If an assay option was selected on the Readme sheet, the relevant information will be highlighted."]
[null,"When performing cell segmentation staining, process two slides per run. The Xenium Stain Enhancer (PN-2000992) cannot be stored once resuspended. It can only be used for one run, regardless of whether one or two slides are processed in a single run. Xenium Protein Subpanel tubes are strictly single-use, regardless of whether one or two slides are\nprocessed. Only retrieve the subpanel tube needed for the experiment. The same subpanel needs to be used for both slides in the same instrument run. Running different subpanel selections in the same instrument run is not supported."]
[]
["Xenium In Situ Gene and Protein Expression with Cell Segmentation Staining (off-instrument; ~3 d; CG000819)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Custom Probe Preparation (optional)",null,null,null,"10 min",null,"-"]
[null,1.3,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 2: Post Hybridization Wash"]
[null,2.1,"Post Hybridization Wash",null,null,null,"35 min"]
[null,"Step 3: Ligation"]
[null,3.1,"Ligation",null,null,null,"~2 h",null,"-"]
[null,"Step 4: Amplification"]
[null,4.0999999999999996,"Amplification",null,null,null,"~ 2 h",null,"-"]
[null,4.2,"Post-Amplification Wash",null,null,null,"15 min",null,{"$error":"#VALUE!"},"4ºC ≤ 2 days"]
[null,"Step 5: Antibody & Cell Segmentation Staining"]
[null,5.0999999999999996,"Buffer Preparation",null,null,null,"20 min",null,"-"]
[null,5.2,"Tissue Dehydration & Secondary Decrosslinking",null,null,null,"40 min"]
[null,5.3,"Block and Stain",null,null,null,"~1 h + 16-24 h (overnight)"]
[null,"Day 3"]
[null,5.4,"Stain Enhancement",null,null,null,"~1 h",null,"-"]
[null,"Step 6: Autofluorescence Quenching"]
[null,6.1,"Autofluorescence Quenching",null,null,null,"30 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,6.2,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]
[]
["Xenium In Situ Gene and Protein Expression Assay Kits, Reagents & Equipment"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene and Protein Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
["Xenium In Situ Gene and Protein Expression with Cell Segmentation Staining (CG000819)"]
[null,"Xenium In Situ Gene and Protein Expression with Cell Segmentation Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Nuclease-free Water\n(select one based on availability)",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[null,null,null,"Nuclease-free Milli-Q water (Biopak® Polisher)\n",null,null,null,"Millipore Sigma",null,"CDUFBI0A1"]
[false,"TE Buffer",null,"TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0",null,null,null,"Fisher Scientific",null,"BP24731"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"TE Buffer, pH 9.0 \n(select one based on availability",null,"Tris EDTA Buffer pH 9.0 (1X), Ready-to-Use, 1000 ml",null,null,null,"Genemed",null,"10-0046"]
[null,null,null,"Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10x), 100 ml",null,null,null,"Biolegend",null,"422703"]
[null,null,null,"Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10x), 500 ml",null,null,null,"Biolegend",null,"422704"]
[null,null,null,"100X Tris-EDTA, pH 9.0",null,null,null,"Abcam",null,"ab93684"]
[null,null,null,"10X Tris-EDTA Buffer, pH 9.0",null,null,null,"Novus",null,"NB900-62085"]
[false,"Glycerol",null,"Glycerol, 99.5 % Molecular Biology, DNAse, RNAse, Protease free (optional, if storing slides long-term)",null,null,null,"Acros Organics",null,"327255000"]
[false,"1.5 ml Tubes",null,"DNA LoBindTubes, 1.5 ml",null,null,null,"Eppendorf",null,22431021]
[null,null,null,"Low DNA Binding Tubes, 1.5 ml",null,null,null,"Sarstedt",null,"72.706.700"]
[false,"2.0 ml Tubes",null,"DNA LoBindTubes, 2.0 ml",null,null,null,"Eppendorf",null,22431048]
[null,null,null,"Low DNA Binding Tubes, 2.0 ml",null,null,null,"Sarstedt",null,"72.695.700"]
[false,"15 ml Tubes",null,"15 ml PP Centrifuge Tubes",null,null,null,"Corning",null,"730791"]
[false,"50 ml Tubes",null,"Self-Standing Polypropylene Centrifuge Tubes, sterile",null,null,null,"Corning",null,"430921"]
[false,"Pipette Tips",null,"Tips LTS 200UL Filter RT-L200 FLR",null,null,"(or equivalent)","Rainin",null,30389240]
[null,null,null,"Tips LTS 1ML Filter RT-L1000 FLR",null,null,null,null,null,30389213]
[null,null,null,"Tips LTS 20UL Filter RT-L20 FLR",null,null,null,null,null,30389226]
[false,"Pipettes",null,"Pipet-Lite LTS Pipette L-20XLS+",null,null,"(or equivalent)","Rainin",null,17014392]
[null,null,null,"Pipet-Lite LTS Pipette L-100XLS+",null,null,null,null,null,17014384]
[null,null,null,"Pipet-Lite LTS Pipette L-200XLS+",null,null,null,null,null,17014391]
[null,null,null,"Pipet-Lite LTS Pipette L-1000XLS+",null,null,null,null,null,17014382]
[false,"Blank Slides",null,"Superfrost Plus Slides (optional, if practicing Xenium Cassette Insert assembly)",null,null,null,"Fisherbrand",null,"12-550-15"]
[false,"Forceps",null,"Fisherbrand Curved Medium Point General Purpose Forceps (or equivalent)",null,null,null,"Fisher Scientific",null,"16-100-110"]
[null,"Additional Materials"]
[false,"Water Bath Alternatively, Eppendorf Thermomixer C (5382000023) with SmartBlock -2.0 mL (5362000035) or equivalent may be used"]
[false,"Mini centrifuge"]
[false,"Vortex"]
[false,"Ice Bucket"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[false,"PCR Sealing Film (optional, for storing slides long-term for Xenium Cassette Insert Practice)"]
[null,"This list may not include some standard laboratory equipment."]

{"sheet":"Xenium Prime Workflow","index":11,"n_rows":76,"n_cols":12,"rows_kept":76,"cut":false,"hidden":false,"merged":[[65,1,65,2],[66,1,66,2],[67,1,67,2],[70,1,70,2],[66,3,66,6],[67,3,67,6],[67,7,67,8],[72,3,72,6],[72,7,72,8],[65,3,65,6],[65,7,65,8],[65,9,65,10],[65,11,65,12],[66,7,66,8],[66,9,66,10],[66,11,66,12],[70,7,70,8],[70,9,70,10],[70,11,70,12],[56,7,56,8],[56,9,56,10],[61,7,64,8],[54,9,54,10],[54,11,54,12],[55,3,55,6],[55,7,55,8],[55,9,55,10],[55,11,55,12],[56,11,56,12],[56,3,56,6],[52,7,52,8],[52,9,52,10],[52,11,52,12],[52,3,52,6],[53,3,53,6],[53,7,53,8],[53,9,53,10],[53,11,53,12],[54,3,54,6],[54,7,54,8],[35,1,35,9],[37,0,37,12],[37,15,37,26],[38,1,38,12],[38,15,38,26],[39,1,39,12],[40,0,40,12],[43,9,43,10],[43,11,43,12],[41,1,41,12],[42,1,42,2],[42,3,42,6],[42,7,42,8],[42,9,42,10],[42,11,42,12],[43,1,44,2],[33,10,33,12],[34,10,34,12],[30,1,30,12],[31,2,31,5],[31,10,31,12],[32,1,32,12],[33,2,33,5],[34,2,34,5],[34,6,34,7],[22,1,22,12],[26,8,26,9],[26,10,26,12],[28,10,28,12],[29,10,29,12],[26,2,26,5],[28,2,28,5],[29,2,29,5],[29,6,29,7],[23,2,23,5],[23,8,23,9],[23,10,23,12],[24,2,24,5],[25,2,25,5],[26,6,26,7],[27,1,27,12],[18,1,18,12],[19,8,19,9],[19,10,19,12],[19,2,19,5],[20,1,20,12],[21,2,21,5],[21,6,21,7],[21,8,21,9],[21,10,21,12],[13,1,13,12],[14,2,14,5],[14,8,14,9],[14,10,14,12],[15,2,15,5],[16,1,16,12],[17,2,17,5],[17,6,17,7],[17,8,17,9],[17,10,17,12],[12,6,12,7],[12,8,12,9],[8,1,8,5],[9,1,9,12],[10,2,10,5],[10,8,10,9],[10,10,10,12],[11,2,11,5],[12,10,12,12],[12,2,12,5],[8,6,8,7],[8,8,8,9],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[8,10,8,12],[68,1,68,12],[69,1,69,12],[63,11,63,12],[64,11,64,12],[57,1,57,2],[58,3,58,5],[58,6,60,6],[58,7,60,8],[58,11,58,12],[59,3,59,5],[59,11,59,12],[61,9,61,10],[63,9,63,10],[60,3,60,5],[60,9,60,10],[61,3,61,5],[61,6,64,6],[74,1,74,10],[74,11,74,12],[75,1,75,12],[70,3,70,6],[71,3,71,6],[71,7,71,8],[71,9,71,10],[71,11,71,12],[73,1,73,10],[73,11,73,12],[71,1,71,2],[72,1,72,2],[72,9,72,10],[72,11,72,12],[62,3,62,5],[63,3,63,5],[64,3,64,5],[64,9,64,10],[58,1,58,2],[60,1,60,2],[61,1,64,2],[58,9,58,10],[59,9,59,10],[57,3,57,6],[57,7,57,8],[57,9,57,10],[57,11,57,12],[60,11,60,12],[61,11,61,12],[50,9,50,10],[50,11,50,12],[46,1,46,2],[47,1,48,2],[49,1,50,2],[51,1,51,2],[52,1,53,2],[54,1,55,2],[56,1,56,2],[47,3,47,6],[47,7,47,8],[47,9,47,10],[47,11,47,12],[48,7,48,8],[48,9,48,10],[48,11,48,12],[48,3,48,6],[49,3,49,6],[51,3,51,6],[43,3,43,6],[43,7,43,8],[44,9,44,10],[44,11,44,12],[45,9,45,10],[45,11,45,12],[46,9,46,10],[46,11,46,12],[44,3,44,6],[44,7,44,8],[51,7,51,8],[51,9,51,10],[51,11,51,12],[45,1,45,2],[45,3,45,6],[45,7,45,8],[46,3,46,6],[46,7,46,8],[49,7,49,8],[49,9,49,10],[49,11,49,12],[50,3,50,6],[50,7,50,8]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Xenium Prime Key Protocol Steps and Timing (off-instrument; for both FFPE & FF samples)"]
[null,"If an assay option was selected on the Readme sheet, the relevant information will be highlighted."]
[null,"When performing cell segmentation staining, process two slides per run. The Xenium Stain Enhancer (PN-2000992) cannot be stored once resuspended. It can only be used for one run, regardless of whether one or two slides are processed in a single run."]
[]
["Xenium Prime In Situ Gene Expression with optional Cell Segmentation Staining (off-instrument; ~3 d; CG000760)"]
[null,"Steps",null,null,null,null,"Timing",null,"Stop & Store",null,"Customer Notes"]
[null,"Day 1\nStep 1: Probe Hybridization"]
[null,1.1000000000000001,"Buffer Hybridization",null,null,null,"20 min",null,"-"]
[null,1.2,"Priming Hybridization",null,null,null,"~1.8 h",null,"-"]
[null,1.3,"Post Priming Hybridization Wash",null,null,null,"70 min",null,"-"]
[null,"Step 2: Post Hybridization Wash"]
[null,2.1,"RNase Treatment",null,null,null,"30 min",null,"-"]
[null,2.2000000000000002,"Polishing",null,null,null,"70 min",null,"-"]
[null,"Step 3: Probe Hybridization"]
[null,3.1,"Probe Hybridization",null,null,null,"16-24 h (overnight)",null,"-"]
[null,"Day 2\nStep 4: Post Hybridization Wash"]
[null,4.0999999999999996,"Post Hybridization Wash",null,null,null,"35 min",null,"-"]
[null,"Step 5: Ligation"]
[null,5.0999999999999996,"Ligation",null,null,null,"40 min",null,"-"]
[null,"Step 6: Amplification"]
[null,6.1,"Amplification Enhancement",null,null,null,"~2 h",null,"-"]
[null,6.2,"Post-Amplification Enhancement Wash",null,null,null,"5 min",null,"-"]
[null,6.3,"Amplification",null,null,null,"~1.6 h",null,"-"]
[null,6.4,"Post-Amplification Wash",null,null,null,"15 min",null,"-"]
[null,"OPTIONAL: Step 7: Cell Segmentation Staining"]
[null,7.1,"Buffer Preparation",null,null,null,"20 min",null,"-"]
[null,7.2,"Block and Stain",null,null,null,"~1 h + 16-24 h (overnight)",null,"-"]
[null,"Day 3"]
[null,7.3,"Stain Enhancement",null,null,null,"~1 h",null,"-"]
[null,"Step 8: Autofluorescence Quenching"]
[null,8.1,"Autofluorescence Quenching",null,null,null,"45 min",null,{"$error":"#VALUE!"},"4ºC overnight (in the dark)"]
[null,8.1999999999999993,"Nuclei Staining",null,null,null,"10 min",null,{"$error":"#VALUE!"},"4ºC overnight or ≤ 1 week (in the dark)"]
[null,"Refer to the relevant user guides for long-term slide storage guidance"]
[]
["Xenium Prime Assay Kits, Reagents & Equipment"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
["Xenium Prime Assay Workflow (CG000760)"]
[null,"Xenium Prime In Situ Gene Expression with Optional Cell Segmentation Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Nuclease-free Water\n(select one based on availability",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[null,null,null,"Nuclease-free Milli-Q water (Biopak® Polisher)\n",null,null,null,"Millipore Sigma",null,"CDUFBI0A1"]
[false,"TE Buffer",null,"TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0",null,null,null,"Fisher Scientific",null,"BP24731"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"Glycerol",null,"Glycerol, 99.5 % Molecular Biology, DNAse, RNAse, Protease free (optional, if storing slides long-term)",null,null,null,"Acros Organics",null,"327255000"]
[false,"1.5 ml Tubes",null,"DNA LoBindTubes, 1.5 ml",null,null,null,"Eppendorf",null,22431021]
[null,null,null,"Low DNA Binding Tubes, 1.5 ml",null,null,null,"Sarstedt",null,"72.706.700"]
[false,"2.0 ml Tubes",null,"DNA LoBindTubes, 2.0 ml",null,null,null,"Eppendorf",null,22431048]
[null,null,null,"Low DNA Binding Tubes, 2.0 ml",null,null,null,"Sarstedt",null,"72.695.700"]
[false,"15 ml Tubes",null,"15 ml PP Centrifuge Tubes",null,null,null,"Corning",null,"730791"]
[false,"50 ml Tubes",null,"Self-Standing Polypropylene Centrifuge Tubes, sterile",null,null,null,"Corning",null,"430921"]
[false,"Pipette Tips",null,"Tips LTS 200UL Filter RT-L200 FLR",null,null,"(or equivalent)","Rainin",null,30389240]
[null,null,null,"Tips LTS 1ML Filter RT-L1000 FLR",null,null,null,null,null,30389213]
[null,null,null,"Tips LTS 20UL Filter RT-L20 FLR",null,null,null,null,null,30389226]
[false,"Pipettes",null,"Pipet-Lite LTS Pipette L-20XLS+",null,null,"(or equivalent)","Rainin",null,17014392]
[null,null,null,"Pipet-Lite LTS Pipette L-100XLS+",null,null,null,null,null,17014384]
[null,null,null,"Pipet-Lite LTS Pipette L-200XLS+",null,null,null,null,null,17014391]
[null,null,null,"Pipet-Lite LTS Pipette L-1000XLS+",null,null,null,null,null,17014382]
[false,"Blank Slides",null,"Superfrost Plus Slides (optional, if practicing Xenium Cassette Insert assembly)",null,null,null,"Fisherbrand",null,"12-550-15"]
[false,"Forceps",null,"Fisherbrand Curved Medium Point General Purpose Forceps (or equivalent)",null,null,null,"Fisher Scientific",null,"16-100-110"]
[false,"SSC Buffer, 20X ",null,"SSC Buffer 20x Concentrate ",null,null,null,"Millipore Sigma",null,"S6639"]
[null,"Additional Materials"]
[false,"Water Bath Alternatively, Eppendorf Thermomixer C (5382000023) with SmartBlock -2.0 mL (5362000035) or equivalent may be used"]
[false,"Mini centrifuge"]
[false,"Vortex"]
[false,"Ice Bucket"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[false,"PCR Sealing Film (optional, for storing slides long-term, for Xenium Cassette Insert Practice)"]
[null,"This list may not include some standard laboratory equipment."]

{"sheet":"Xenium Analyzer","index":12,"n_rows":80,"n_cols":16,"rows_kept":80,"cut":false,"hidden":false,"merged":[[67,9,67,10],[67,11,67,12],[68,7,68,8],[68,9,68,10],[68,11,68,12],[68,3,68,6],[69,3,69,6],[69,7,69,8],[69,9,69,10],[69,11,69,12],[25,0,25,12],[27,1,28,3],[27,4,27,9],[27,10,28,12],[28,4,28,9],[53,9,53,10],[53,11,53,12],[57,7,57,8],[58,7,58,8],[58,9,58,10],[58,11,58,12],[50,1,50,12],[51,3,51,6],[51,7,51,8],[51,9,51,10],[51,11,51,12],[43,1,43,11],[45,1,45,11],[46,1,46,12],[47,0,47,12],[48,1,48,12],[49,1,49,12],[32,10,32,12],[33,1,33,11],[19,2,19,5],[19,8,19,9],[19,10,19,12],[20,2,20,5],[20,6,20,7],[20,8,20,9],[20,10,20,12],[22,0,22,12],[23,1,23,12],[13,2,13,4],[14,8,14,9],[14,2,14,4],[15,2,15,4],[15,8,15,9],[16,2,16,4],[17,2,17,4],[17,8,17,9],[18,1,18,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[7,0,7,13],[8,1,8,5],[8,6,8,7],[8,8,8,9],[8,10,8,12],[9,1,9,12],[10,8,10,9],[10,10,10,12],[10,2,10,5],[11,1,11,12],[12,2,12,5],[12,8,12,9],[12,10,12,12],[42,1,42,3],[42,4,42,9],[42,10,42,12],[41,4,41,9],[41,10,41,12],[39,1,39,3],[39,4,39,9],[39,10,39,12],[40,1,40,3],[40,4,40,9],[40,10,40,12],[41,1,41,3],[37,4,37,9],[38,4,38,9],[32,1,32,3],[32,4,32,9],[35,0,35,12],[37,1,38,3],[37,10,38,12],[31,4,31,9],[31,10,31,12],[29,1,29,3],[29,4,29,9],[29,10,29,12],[30,1,30,3],[30,4,30,9],[30,10,30,12],[31,1,31,3],[75,1,75,9],[76,1,76,10],[77,1,77,10],[78,1,78,10],[78,11,78,12],[79,1,79,12],[80,1,80,12],[70,3,70,6],[71,3,71,6],[71,7,71,8],[71,9,71,10],[72,3,72,6],[73,1,73,9],[74,1,74,9],[70,7,70,8],[70,9,70,10],[71,1,71,2],[72,1,72,2],[56,3,56,6],[57,3,57,6],[58,3,58,6],[59,3,59,6],[60,3,60,6],[61,3,61,6],[62,3,62,6],[67,3,67,6],[67,1,67,2],[68,1,68,2],[59,7,59,8],[67,7,67,8],[64,3,64,6],[65,3,65,6],[65,7,65,8],[69,1,69,2],[70,1,70,2],[60,7,60,8],[61,7,61,8],[62,7,62,8],[63,7,63,8],[51,1,51,2],[52,1,52,2],[52,3,52,6],[52,7,52,8],[52,9,52,10],[52,11,52,12],[53,1,54,2],[54,3,54,6],[54,7,54,8],[53,3,53,6],[53,7,53,8],[54,9,54,10],[54,11,54,12],[65,9,65,10],[65,11,65,12],[66,1,66,12],[55,9,55,10],[55,11,55,12],[56,9,56,10],[56,11,56,12],[57,9,57,10],[57,11,57,12],[63,9,63,10],[63,11,63,12],[63,3,63,6],[55,1,55,2],[55,3,55,6],[55,7,55,8],[56,1,57,2],[56,7,56,8],[58,1,62,2],[63,1,65,2],[59,9,59,10],[60,9,60,10],[61,9,61,10],[62,9,62,10],[62,11,62,12]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Xenium Analyzer Key Protocol Steps and Timing (on-instrument; for both FFPE & FF samples)"]
[null,"If an assay option was selected on the Readme sheet, the relevant information will be highlighted.",null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"When performing cell segmentation staining, process two slides per run. The Xenium Stain Enhancer (PN-2000992) cannot be stored once resuspended. It can only be used for one run, regardless of whether one or two slides are processed in a single run."]
[]
["Xenium Analyzer (on-instrument; Xenium v1: ~2-4 d; Xenium Prime and Xenium Protein: ~2-6 d; CG000584)"]
[null,"Steps",null,null,null,null,"Hands-on Time",null,"Total Time",null,"Customer Notes"]
[null,"Day 1"]
[null,null,"Thaw Decoding Reagents",null,null,null,"5 min",null,"16-24 h (overnight)"]
[null,"Day 2"]
[null,null,"Prepare Buffers",null,null,null,"35 min",null,"1 h"]
[null,null,"Initialize Instrument",null,null,null,"-"]
[null,null,"Input Experimental Details",null,null,null,"5-10 min",null,"5-10 min"]
[null,null,"Load Instrument",null,null,null,"~5 min",null,"~5 min"]
[null,null,"Overview Scan",null,null,null,"-",null,"1 h"]
[null,null,"Select Region & Initiate Run",null,null,null,"~10 min",null,"~10 min"]
[null,"Day 2+"]
[null,null,"Run Time",null,null,null,"-",null,"Xenium v1: ~2-4 days\nXenium Prime: ~2-6 days\nXenium Protein: ~2-6 days"]
[null,null,"Post-Run Cleanup",null,null,null,"5 min",null,"10 min"]
[]
["Xenium Analyzer Bulk Reagents"]
[null,"Xenium Analyzer runs require bulk reagents (≥100 ml). Approximate volumes for two Xenium Slides per Xenium instrument run are listed below. Precise volumes for all instrument related reagents are found in the Xenium Analyzer User Guide. For reagent volumes needed for sample preparation and assay workflow, consult the buffer preparation sections of the relevant protocols and user guides."]
[]
["Xenium v1 Workflows: Volume of Bulk Reagents",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(OR(P5=\"Xenium_v1\",P5=\"Xenium_v1_Cell_Seg\"),\"Y\",\"N\")","v":"N"}]
[null,"Xenium v1: Volume of Bulk Reagents (≥100 ml)"]
[null,"Item",null,null,"Approx. Volumes for two Xenium slides",null,null,null,null,null,"Customer Notes"]
[null,null,null,null,"Instrument Run Only"]
[null,"Nuclease-free Water",null,null,"1,140 ml*"]
[null,"Ultrapure Water/Milli-Q Water",null,null,"1,000 ml"]
[null,"10X PBS",null,null,"100 ml"]
[null,"100% DMSO",null,null,"150 ml"]
[null,"*For instrument run ONLY: nuclease-free water can be substituted with nuclease-free Ultrapure/Milli-Q water."]
[]
["Xenium Prime and Xenium Protein Workflows: Volume of Bulk Reagents",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(OR(P5=\"Xenium_Prime\",P5=\"Xenium_Protein\"),\"Y\",\"N\")","v":"Y"}]
[null,"Xenium Prime: Volume of Bulk Reagents (≥100 ml)"]
[null,"Item",null,null,"Approx. Volumes for two Xenium slides",null,null,null,null,null,"Customer Notes"]
[null,null,null,null,"Instrument Run Only"]
[null,"Nuclease-free Water",null,null,"2,233 ml*"]
[null,"Ultrapure Water/Milli-Q Water",null,null,"500 ml"]
[null,"10X PBS",null,null,"200 ml"]
[null,"100% DMSO",null,null,"250 ml"]
[null,"*For instrument run ONLY: nuclease-free water can be substituted with nuclease-free Ultrapure/Milli-Q water."]
[]
[null,"For instrument run during installation and also during training, twice the amount of indicated reagent volumes are needed."]
[]
["Xenium Analyzer Kits, Reagents & Equipment"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
[null,"For Reagent Bottle Buffer Preparation"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Nuclease-free Water",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[false,"PBS-T\n(select one based on availability",null,"Phosphate Buffered Saline with 0.05% Tween 20, pH 7.4",null,null,null,"Millipore Sigma",null,"P3563-10PAK"]
[null,null,null,"Phosphate Buffered Saline with 0.05% Tween 20, pH 7.4",null,null,null,"Millipore Sigma",null,"PPB005-20PAK"]
[false,"PBS (alternate for making PBS-T)",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution) (use one ampule per use)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"100% DMSO\n(select one based on availability",null,"Dimethyl sulfoxide (molecular biology grade)",null,null,null,"Millipore Sigma",null,"41639-500 ML"]
[null,null,null,"Dimethyl sulfoxide (molecular biology grade)",null,null,null,"Millipore Sigma",null,"D8418-250ML"]
[null,null,null,"Dimethyl sulfoxide (molecular biology grade)",null,null,null,"Millipore Sigma",null,"D8418-1L"]
[null,null,null,"Dimethyl sulfoxide, Fisher BioReagents (>99.7%)",null,null,null,"Fisher Scientific",null,"BP231-1"]
[null,null,null,"Dimethyl sulfoxide (for molecular biology, 99.5+%)",null,null,null,"Fuji Film",null,"043-29355 500 ml"]
[false,"KCl\n(select one based on availability)",null,"Potassium Chloride (KCl, sterile), 500 ml",null,null,null,"Teknova",null,"P0330"]
[null,null,null,"Potassium Chloride (KCl, sterile), 1 L",null,null,null,"Teknova",null,"P0335"]
[null,null,null,"KCl (2 M), RNase-free ",null,null,null,"Invitrogen",null,"AM9640G"]
[null,"Additional Materials"]
[false,"Centrifuge",null,"Allegra X-14 Series Benchtop Centrifuge 120 V or equivalent; fits deep-well 96 well plates (~2 ml vol.)",null,null,null,"Beckman Coulter",null,"-"]
[false,"Serological Pipettes",null,"10 ml, 25 ml, 50 ml, 100 ml"]
[false,"Serological Pipettes Controller",null,"Compatible with 10, 25, 50 & 100 ml serological pipettes"]
[false,"Pipette Tips",null,"Tips LTS 1ML Filter RT-L1000FLR (or equivalent)",null,null,null,"Rainin",null,30389213]
[false,"Pipettes",null,"Pipet-Lite LTS Pipette L-1000XLS+ (or equivalent)",null,null,null,"Rainin",null,17014382]
[false,"Glass Bottles with Cap",null,"Pyrex Reusable Media Storage Bottles (500 ml and 1 L)\n(or equivalent)"]
[false,"Compressed Canned Air for cleaning"]
[false,"Lens-cleaning Paper or Lint-free Laboratory Wipes"]
[false,"Plate Seal"]
[false,"70% Isopropanol"]
[false,"Laboratory Balance"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]

{"sheet":"Assay Kits & Reagents","index":13,"n_rows":72,"n_cols":16,"rows_kept":72,"cut":false,"hidden":true,"merged":[[39,1,39,10],[39,11,39,12],[40,1,40,10],[40,11,40,12],[41,1,41,12],[42,1,42,12],[37,1,37,2],[38,1,38,2],[26,1,26,2],[26,3,26,5],[27,3,27,5],[27,6,30,6],[27,7,30,8],[28,3,28,5],[29,3,29,5],[30,3,30,5],[31,3,31,6],[31,7,31,8],[32,3,32,6],[32,7,32,8],[37,3,37,6],[37,7,37,8],[37,9,37,10],[37,11,37,12],[36,11,36,12],[36,3,36,6],[24,1,24,2],[24,3,24,5],[24,6,26,6],[24,7,26,8],[24,9,24,10],[24,11,24,12],[25,3,25,5],[27,1,27,2],[30,1,30,2],[25,9,25,10],[25,11,25,12],[33,1,33,2],[36,1,36,2],[23,9,23,10],[23,11,23,12],[38,3,38,6],[38,7,38,8],[26,9,26,10],[26,11,26,12],[27,9,27,10],[27,11,27,12],[29,9,29,10],[29,11,29,12],[30,9,30,10],[30,11,30,12],[32,9,32,10],[32,11,32,12],[31,9,31,10],[31,11,31,12],[38,9,38,10],[38,11,38,12],[33,3,33,6],[33,7,33,8],[34,1,34,12],[35,1,35,12],[36,7,36,8],[36,9,36,10],[20,1,21,2],[20,3,20,6],[21,3,21,6],[22,1,22,2],[22,3,22,6],[21,7,21,8],[22,7,22,8],[31,1,31,2],[32,1,32,2],[23,1,23,2],[23,3,23,6],[23,7,23,8],[22,9,22,10],[22,11,22,12],[19,7,19,8],[19,9,19,10],[20,7,20,8],[20,9,20,10],[20,11,20,12],[21,9,21,10],[21,11,21,12],[18,7,18,8],[18,9,18,10],[17,1,17,2],[17,3,17,6],[17,7,17,8],[17,9,17,10],[17,11,17,12],[18,1,19,2],[18,11,18,12],[19,11,19,12],[18,3,18,6],[19,3,19,6],[13,1,14,2],[15,1,16,2],[16,3,16,6],[16,7,16,8],[16,9,16,10],[16,11,16,12],[14,3,14,6],[15,3,15,6],[15,7,15,8],[15,9,15,10],[15,11,15,12],[13,3,13,6],[13,7,13,8],[13,9,13,10],[13,11,13,12],[14,7,14,8],[14,9,14,10],[14,11,14,12],[11,1,11,2],[11,3,11,6],[11,7,11,8],[10,9,10,10],[10,11,10,12],[11,9,11,10],[11,11,11,12],[12,3,12,6],[12,7,12,8],[12,1,12,2],[12,9,12,10],[12,11,12,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[6,0,6,12],[9,3,9,6],[9,7,9,8],[9,9,9,10],[9,11,9,12],[7,1,7,12],[8,1,8,2],[8,3,8,6],[8,7,8,8],[8,9,8,10],[8,11,8,12],[9,1,10,2],[10,3,10,6],[10,7,10,8]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Xenium Assay Kits, Reagents & Equipment",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
["Xenium Assay Workflow (CG000582, CG000749, or CG000760)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P4=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]
[null,"Xenium In Situ Gene Expression"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[null,"Nuclease-free Water\n(select one based on availability",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[null,null,null,"Nuclease-free Milli-Q water (Biopak® Polisher)\n",null,null,null,"Millipore Sigma",null,"CDUFBI0A1"]
[null,"TE Buffer",null,"TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0",null,null,null,"Fisher Scientific",null,"BP24731"]
[null,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4,\nRNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[null,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[null,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[null,"Glycerol",null,"Glycerol, 99.5 % Molecular Biology, DNAse, RNAse, Protease free (optional, if storing slides long-term)",null,null,null,"Acros Organics",null,"327255000"]
[null,"1.5 ml Tubes",null,"DNA LoBindTubes, 1.5 ml",null,null,null,"Eppendorf",null,22431021]
[null,null,null,"Low DNA Binding Tubes, 1.5 ml",null,null,null,"Sarstedt",null,"72.706.700"]
[null,"2.0 ml Tubes",null,"DNA LoBindTubes, 2.0 ml",null,null,null,"Eppendorf",null,22431048]
[null,null,null,"Low DNA Binding Tubes, 2.0 ml",null,null,null,"Sarstedt",null,"72.695.700"]
[null,"15 ml Tubes",null,"15 ml PP Centrifuge Tubes",null,null,null,"Corning",null,"730791"]
[null,"50 ml Tubes",null,"Self-Standing Polypropylene Centrifuge Tubes, sterile",null,null,null,"Corning",null,"430921"]
[null,"Pipette Tips",null,"Tips LTS 200UL Filter RT-L200 FLR",null,null,"(or equivalent)","Rainin",null,30389240]
[null,null,null,"Tips LTS 1ML Filter RT-L1000 FLR",null,null,null,null,null,30389213]
[null,null,null,"Tips LTS 20UL Filter RT-L20 FLR",null,null,null,null,null,30389226]
[null,"Pipettes",null,"Pipet-Lite LTS Pipette L-20XLS+",null,null,"(or equivalent)","Rainin",null,17014392]
[null,null,null,"Pipet-Lite LTS Pipette L-100XLS+",null,null,null,null,null,17014384]
[null,null,null,"Pipet-Lite LTS Pipette L-200XLS+",null,null,null,null,null,17014391]
[null,null,null,"Pipet-Lite LTS Pipette L-1000XLS+",null,null,null,null,null,17014382]
[null,"Blank Slides",null,"Superfrost Plus Slides (optional, if practicing Xenium Cassette Insert assembly)",null,null,null,"Fisherbrand",null,"12-550-15"]
[null,"Forceps",null,"Fisherbrand Curved Medium Point General Purpose Forceps (or equivalent)",null,null,null,"Fisher Scientific",null,"16-100-110"]
[null,"SSC Buffer, 20X \n(only for Xenium Prime)",null,"SSC Buffer 20x Concentrate (only for Xenium Prime)",null,null,null,"Millipore Sigma",null,"S6639"]
[null,"Additional Materials"]
[null,"Water Bath Alternatively, Eppendorf Thermomixer C (5382000023) with SmartBlock -2.0 mL (5362000035) or equivalent may be used"]
[null,"Mini centrifuge"]
[null,"Vortex"]
[null,"Ice Bucket"]
[null,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"PCR Sealing Film (optional, for storing slides long-term, for Xenium Cassette Insert Practice)"]
[null,"This list may not include some standard laboratory equipment."]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P69=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]

{"sheet":"Instrument Kits & Reagents","index":14,"n_rows":74,"n_cols":16,"rows_kept":74,"cut":false,"hidden":true,"merged":[[40,11,40,12],[34,1,34,2],[34,3,34,6],[34,7,34,8],[34,9,34,10],[34,11,34,12],[35,1,36,2],[35,11,35,12],[36,11,36,12],[38,7,38,8],[39,7,39,8],[39,9,39,10],[39,11,39,12],[37,7,37,8],[37,9,37,10],[37,11,37,12],[38,9,38,10],[38,11,38,12],[33,11,33,12],[36,7,36,8],[36,9,36,10],[29,0,29,12],[30,1,30,12],[31,1,31,12],[32,1,32,12],[33,1,33,2],[33,3,33,6],[33,7,33,8],[35,7,35,8],[35,9,35,10],[53,1,53,2],[53,3,53,6],[53,7,53,8],[53,9,53,10],[51,1,51,2],[51,3,51,6],[51,7,51,8],[51,9,51,10],[51,11,51,12],[52,1,52,2],[52,9,52,10],[52,3,52,6],[52,7,52,8],[44,11,44,12],[45,7,45,8],[45,9,45,10],[45,11,45,12],[54,1,54,2],[60,1,60,10],[61,1,61,12],[62,1,62,12],[54,3,54,6],[55,1,55,9],[56,1,56,9],[57,1,57,9],[58,1,58,10],[59,1,59,10],[60,11,60,12],[47,7,47,8],[47,9,47,10],[47,11,47,12],[48,1,48,12],[50,7,50,8],[50,9,50,10],[49,1,49,2],[49,3,49,6],[49,7,49,8],[49,9,49,10],[49,11,49,12],[50,3,50,6],[50,11,50,12],[50,1,50,2],[25,1,25,8],[27,1,27,11],[28,1,28,12],[41,3,41,6],[42,3,42,6],[43,3,43,6],[44,3,44,6],[45,1,47,2],[45,3,45,6],[46,3,46,6],[47,3,47,6],[35,3,35,6],[36,3,36,6],[37,1,37,2],[37,3,37,6],[38,1,39,2],[38,3,38,6],[40,1,44,2],[42,7,42,8],[42,9,42,10],[43,7,43,8],[43,9,43,10],[44,7,44,8],[44,9,44,10],[22,1,22,3],[22,4,22,6],[22,7,22,8],[23,4,23,6],[23,7,23,8],[23,1,23,3],[24,1,24,3],[24,4,24,6],[24,7,24,8],[33,9,33,10],[39,3,39,6],[40,3,40,6],[40,7,40,8],[40,9,40,10],[41,7,41,8],[41,9,41,10],[15,1,15,8],[17,0,17,12],[19,1,20,3],[19,4,19,6],[19,7,20,8],[20,4,20,6],[21,1,21,3],[21,4,21,6],[21,7,21,8],[11,1,11,3],[11,4,11,6],[11,7,11,8],[14,4,14,6],[14,7,14,8],[12,1,12,3],[12,4,12,6],[12,7,12,8],[13,1,13,3],[13,4,13,6],[13,7,13,8],[14,1,14,3],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[7,0,7,12],[9,1,10,3],[9,4,9,6],[9,7,10,8],[10,4,10,6]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Xenium Analyzer Bulk Reagents",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"If a workflow option was selected on the Readme sheet, the relevant information will be highlighted."]
[null,"Xenium Analyzer runs require bulk reagents (≥100 ml). Approximate volumes for two Xenium Slides per Xenium instrument run are listed below. Precise volumes for all instrument related reagents are found in the Xenium Analyzer User Guide. For reagent volumes needed for sample preparation and assay workflow, consult the buffer preparation sections of the relevant protocols and user guides."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P4=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]
["Xenium v1 Workflows: Volume of Bulk Reagents"]
[null,"Xenium v1: Volume of Bulk Reagents (≥100 ml)"]
[null,"Item",null,null,"Approx. Volumes for two Xenium slides",null,null,"Customer Notes"]
[null,null,null,null,"Instrument Run Only"]
[null,"Nuclease-free Water",null,null,"1,140 ml*"]
[null,"Ultrapure Water/Milli-Q Water",null,null,"1,000 ml"]
[null,"10X PBS",null,null,"100 ml"]
[null,"100% DMSO",null,null,"150 ml"]
[null,"*For instrument run ONLY: nuclease-free water can be substituted with nuclease-free Ultrapure/Milli-Q water."]
[]
["Xenium Prime Workflows: Volume of Bulk Reagents"]
[null,"Xenium Prime: Volume of Bulk Reagents (≥100 ml)"]
[null,"Item",null,null,"Approx. Volumes for two Xenium slides",null,null,"Customer Notes"]
[null,null,null,null,"Instrument Run Only"]
[null,"Nuclease-free Water",null,null,"2,233 ml*"]
[null,"Ultrapure Water/Milli-Q Water",null,null,"500 ml"]
[null,"10X PBS",null,null,"200 ml"]
[null,"100% DMSO",null,null,"250 ml"]
[null,"*For instrument run ONLY: nuclease-free water can be substituted with nuclease-free Ultrapure/Milli-Q water."]
[]
[null,"For instrument run during installation and also during training, twice the amount of indicated reagent volumes are needed."]
[]
["Xenium Analyzer Kits, Reagents & Equipment"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available."]
[]
[null,"For Reagent Bottle Buffer Preparation"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[null,"Nuclease-free Water",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[null,"PBS-T\n(select one based on availability",null,"Phosphate Buffered Saline with 0.05% Tween 20, pH 7.4",null,null,null,"Millipore Sigma",null,"P3563-10PAK"]
[null,null,null,"Phosphate Buffered Saline with 0.05% Tween 20, pH 7.4",null,null,null,"Millipore Sigma",null,"PPB005-20PAK"]
[null,"PBS (alternate for making PBS-T)",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4,\nRNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[null,"10% Tween 20",null,"Tween 20 Surfact-Amps Detergent Solution (10%\nsolution) (use one ampule per use)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[null,"100% DMSO\n(select one based on availability",null,"Dimethyl sulfoxide (molecular biology grade)",null,null,null,"Millipore Sigma",null,"41639-500 ML"]
[null,null,null,"Dimethyl sulfoxide (molecular biology grade)",null,null,null,"Millipore Sigma",null,"D8418-250ML"]
[null,null,null,"Dimethyl sulfoxide (molecular biology grade)",null,null,null,"Millipore Sigma",null,"D8418-1L"]
[null,null,null,"Dimethyl sulfoxide, Fisher BioReagents (>99.7%)",null,null,null,"Fisher Scientific",null,"BP231-1"]
[null,null,null,"Dimethyl sulfoxide (for molecular biology, 99.5+%)",null,null,null,"Fuji Film",null,"043-29355 500 ml"]
[null,"KCl\n(select one based on availability)",null,"Potassium Chloride (KCl, sterile), 500 ml",null,null,null,"Teknova",null,"P0330"]
[null,null,null,"Potassium Chloride (KCl, sterile), 1 L",null,null,null,"Teknova",null,"P0335"]
[null,null,null,"KCl (2 M), RNase-free ",null,null,null,"Invitrogen",null,"AM9640G"]
[null,"Additional Materials"]
[null,"Centrifuge",null,"Allegra X-14 Series Benchtop Centrifuge 120 V or equivalent; fits deep-well 96 well plates (~2 ml vol.)",null,null,null,"Beckman Coulter",null,"-"]
[null,"Serological Pipettes",null,"10 ml, 25 ml, 50 ml, 100 ml"]
[null,"Serological Pipettes Controller",null,"Compatible with 10, 25, 50 & 100 ml serological pipettes"]
[null,"Pipette Tips",null,"Tips LTS 1ML Filter RT-L1000FLR (or equivalent)",null,null,null,"Rainin",null,30389213]
[null,"Pipettes",null,"Pipet-Lite LTS Pipette L-1000XLS+ (or equivalent)",null,null,null,"Rainin",null,17014382]
[null,"Glass Bottles with Cap",null,"Pyrex Reusable Media Storage Bottles (500 ml and 1 L)\n(or equivalent)"]
[null,"Compressed Canned Air for cleaning"]
[null,"Lens-cleaning Paper or Lint-free Laboratory Wipes"]
[null,"Plate Seal"]
[null,"70% Isopropanol"]
[null,"Laboratory Balance"]
[null,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P71=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]

{"sheet":"Post-run H&E Kits & Reagents","index":15,"n_rows":77,"n_cols":16,"rows_kept":77,"cut":false,"hidden":false,"merged":[[36,1,36,12],[37,1,37,12],[25,9,25,10],[25,11,25,12],[26,3,26,6],[26,7,26,8],[34,3,34,6],[34,7,34,8],[34,9,34,10],[34,11,34,12],[35,1,35,10],[35,11,35,12],[33,9,33,10],[33,11,33,12],[30,9,30,10],[30,11,30,12],[29,9,29,10],[29,11,29,12],[30,3,30,6],[30,7,30,8],[21,1,22,2],[23,1,24,2],[27,1,27,2],[28,1,28,2],[33,1,33,2],[34,1,34,2],[19,1,19,2],[20,1,20,2],[20,3,20,6],[21,3,21,6],[23,3,23,6],[24,3,24,6],[25,3,25,5],[31,1,31,12],[32,1,32,10],[32,11,32,12],[33,3,33,6],[33,7,33,8],[28,7,28,8],[28,9,28,10],[28,11,28,12],[28,3,28,6],[29,3,29,6],[29,7,29,8],[16,9,16,10],[16,11,16,12],[19,9,19,10],[21,7,21,8],[21,9,21,10],[27,3,27,6],[27,7,27,8],[27,9,27,10],[27,11,27,12],[22,3,22,6],[22,7,22,8],[22,11,22,12],[20,7,20,8],[20,9,20,10],[22,9,22,10],[23,7,23,8],[23,9,23,10],[23,11,23,12],[24,7,24,8],[24,9,24,10],[24,11,24,12],[26,9,26,10],[26,11,26,12],[25,7,25,8],[15,9,15,10],[15,11,15,12],[11,3,11,6],[11,7,11,8],[12,1,12,2],[12,3,12,6],[12,7,12,8],[14,1,14,12],[15,1,15,2],[15,3,15,6],[15,7,15,8],[12,11,12,12],[11,9,11,10],[11,11,11,12],[12,9,12,10],[9,1,9,2],[10,1,10,2],[10,3,10,6],[10,7,10,8],[10,9,10,10],[10,11,10,12],[11,1,11,2],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[5,1,5,12],[7,1,7,12],[9,7,9,8],[9,9,9,10],[8,1,8,2],[8,3,8,6],[8,7,8,8],[8,9,8,10],[8,11,8,12],[9,3,9,6],[9,11,9,12],[17,3,17,6],[17,7,17,8],[18,3,18,6],[18,7,18,8],[16,1,16,2],[16,3,16,6],[16,7,16,8],[19,3,19,6],[19,7,19,8]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Quencher Removal & H&E Staining - Reagents & Consumables (not supplied by 10x Genomics)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"Optional; Only if following 10x Genomics H&E protocol (CG000613)"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available. Refer to the manufacturer's website for regional part numbers."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P4=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]
[null,"For Quencher Removal (Post-run quencher removal is required only if staining slides after the instrument run)"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Sodium Hydrosulfite",null,"Sodium hydrosulfite, technical grade (or equivalent)",null,null,null,"Sigma Aldrich",null,"157953-5G"]
[false,"Forceps",null,"Tweezers, 4' Water Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"PBS (optional)",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4,\nRNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"Slide Mailers",null,"Sim port Scientific LockMailer Tamper Evident Slide\nMailer",null,null,null,"Fisher Scientific",null,"22-038-399"]
[]
[null,"For H&E Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Hematoxylin",null,"Hematoxylin Solution, Mayer's",null,null,null,"Sigma Aldrich",null,"MHS16"]
[null,null,null,"EMS Mayer's Hematoxylin",null,null,null,"Electron Microscopy Sciences",null,"26043-05"]
[null,null,null,"Hematoxylin, Mayer's, Classical",null,null,null,"Ethos Biosciences",null,"7021"]
[false,"Eosin",null,"Eosin Y Solution, Alcoholic",null,null,null,"Leica",null,3801615]
[false,"Bluing Reagent",null,"Bluing Solution",null,null,null,"Dako",null,"CS702"]
[false,"Mounting Media",null,"Surgipath SUB-X Mounting Media Discontd.",null,null,null,"Leica",null,3801741]
[null,null,null,"Epredia Cytoseal Mountant\nCytoseal or equivalent mounting media can be used",null,null,null,"Fisher Scientific",null,"22-050-262"]
[false,"Ethanol",null,"Ethyl Alcohol, Pure (200 Proof, anhydrous)",null,null,null,"Millipore Sigma",null,"E7023"]
[null,null,null,"Ethanol absolute ≥99.5%, TechniSolv, pure \n(Europe Only)",null,null,null,"VWR",null,"83813.360DP"]
[false,"Xylene",null,"Xylene, Reagent Grade",null,null,null,"Millipore Sigma",null,214736]
[null,null,null,"Xylene, Histological Grade",null,null,null,"Millipore Sigma",null,534056]
[false,"Forceps",null,"Tweezers, 4' Water Handling",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"Filter Paper",null,"Fisherbrand Qualitative Grade Plain Filter Paper Circles",null,null,null,"Fisher Scientific",null,"09-795-H"]
[false,"Coverslips",null,"Fisherbrand Cover Glasses: Rectangles (Discontinued)",null,null,null,"Fisher Scientific",null,"12-544-EP"]
[null,null,null,"Cover Glasses, Rectangles",null,null,null,"VWR",null,"16004-322"]
[null,"Additional Materials"]
[false,"Vortex"]
[false,"Staining jar/dishes"]
[false,"Wide-bore pipette tips"]
[false,"Ultrapure/Milli-Q Water for Water Bath, from Milli-Q Integral Ultrapure Water System or equivalent"]
[null,"This list may not include some standard laboratory equipment."]
[]
[]
[]
[]
[]
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[]
[]
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[]
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{"sheet":"Post-run IF Kits & Reagents","index":16,"n_rows":63,"n_cols":16,"rows_kept":63,"cut":false,"hidden":false,"merged":[[5,1,5,12],[0,1,0,12],[1,1,1,12],[2,1,2,12],[3,0,3,12],[4,1,4,12],[9,11,9,12],[7,1,7,12],[8,1,8,2],[8,3,8,6],[8,7,8,8],[8,9,8,10],[8,11,8,12],[10,1,10,2],[10,3,10,6],[10,7,10,8],[10,9,10,10],[9,1,9,2],[9,3,9,6],[9,7,9,8],[9,9,9,10],[11,3,11,6],[11,7,11,8],[11,9,11,10],[12,3,12,6],[12,7,12,8],[12,9,12,10],[13,1,13,2],[13,3,13,6],[13,7,13,8],[13,9,13,10],[13,11,13,12],[15,3,15,6],[15,7,15,8],[15,9,15,10],[15,11,15,12],[14,3,14,5],[14,7,14,8],[14,9,14,10],[14,11,14,12],[22,1,22,12],[23,1,23,12],[11,1,12,2],[14,1,14,2],[16,1,20,2],[16,3,16,6],[17,3,17,6],[18,3,18,6],[21,1,21,2],[21,3,21,6],[21,7,21,8],[21,9,21,10],[21,11,21,12],[19,3,19,6],[20,3,20,6],[15,1,15,2]]}
[null,"PROTOCOL PLANNER  |  CG000601 Rev G  |  10xgenomics.com"]
[null,"Xenium In Situ Gene Expression"]
[]
["Quencher Removal & H&E Staining - Reagents & Consumables (not supplied by 10x Genomics)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"Readme!D31","v":"Xenium_Protein"}]
[null,"Optional; Only if following 10x Genomics Post-Xenium IF protocol (CG000709)"]
[null,"The items in the tables below have been tested by 10x Genomics and perform optimally with Xenium In Situ Gene Expression protocols. Substituting materials may adversely affect system performance. This list may not include standard laboratory equipment such as water baths, centrifuges, pH meters, freezers etc. For items with multiple options listed, choose option based on availability and preference. Alternative part numbers for different volumes of the same validated reagent may be available. Refer to the manufacturer's website for regional part numbers."]
[]
[null,"For H&E Staining"]
[null,"Item",null,"Description",null,null,null,"Supplier",null,"Part Number (US)",null,"Customer Notes"]
[false,"Nuclease-free Water",null,"Nuclease-free water (not-DEPC treated)",null,null,null,"Thermo Fisher Scientific",null,"AM9932/AM9937"]
[false,"PBS",null,"PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free",null,null,null,"Thermo Fisher Scientific",null,"AM9624"]
[false,"10% Tween",null,"Tween 20 Surfact-Amps Detergent Solution (10% solution)\n(not 100% Tween diluted to 10%)",null,null,null,"Thermo Fisher Scientific",null,28320]
[null,null,null,"10% Tween-20",null,null,null,"Bio-Rad",null,"1662404/1610781"]
[false,"FBS",null,"Fetal Bovine Serum, qualified, heat inactivated",null,null,null,"Thermo Fisher Scientific",null,"16140-071"]
[false,"Dextran Sulfate",null,"Dextran Sulfate Sodium Salt",null,null,null,"Millipore Sigma",null,"42867"]
[false,"Citrate Buffer",null,"Citrate Buffer, pH 6.0, 10X, Antigen Retriever\n(dlilute 10-fold with water for 1X working solution)",null,null,null,"Excelta Corp",null,"491P-SA-PI"]
[false,"TE Buffer, pH 9.0 \n(select one based on availability",null,"Tris EDTA Buffer pH 9.0 (1X), Ready-to-Use, 1000 ml",null,null,null,"Genemed",null,"10-0046"]
[null,null,null,"Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10x), 100 ml",null,null,null,"Biolegend",null,"422703"]
[null,null,null,"Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10x), 500 ml",null,null,null,"Biolegend",null,"422704"]
[null,null,null,"100X Tris-EDTA, pH 9.0",null,null,null,"Abcam",null,"ab93684"]
[null,null,null,"10X Tris-EDTA Buffer, pH 9.0",null,null,null,"Novus",null,"NB900-62085"]
[false,"Blank Slides",null,"Superfrost Plus Slides",null,null,null,"Fisherbrand",null,"12-550-15"]
[null,"This list may not include some standard laboratory equipment."]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
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[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,{"$f":"IF(P60=\"Fresh Frozen – GEX\",\"Y\",\"N\")","v":"N"}]

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