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[]
[null,null,null,"Chromium Connect",null,null,null,null,null,"Doc","CG000435"]
[null,null,null,"Single Cell 3' Gene Expression Input File ",null,null,null,null,null,"Rev","D"]
[]
["Scope"]
[null,"Use this spreadsheet to save and export your Setup selections (to a csv file) for the Single Cell 3' Gene Expression v3.1 assay on the Chromium Connect Instrument. If you have your own LIMS system, use CG000309 instead."]
[]
["Associated Documents"]
[null,"Refer to",null,"Description"]
[null,"CG000180",null,"Chromium Connect Instrument User Guide"]
[null,"CG000286",null,"Chromium Next GEM Automated Single Cell 3' Reagent Kits v3.1 User Guide"]
[null,"CG000309",null,"ChromiumConnect_SC3-GEX_InputSampleInfo_Template.csv"]
[]
["Required Information"]
[null,"1.","Run Parameters"]
[null,"2.","Sample IDs"]
[null,"3.","Sample Index choices"]
[null,"4.","Cell Count"]
[null,"5.","Input Type"]
[null,"6.","Expression Level"]
[null,"7.","cDNA Input Volume (GEX Library Construction only)"]
[]
["Considerations"]
[null,"•","The experiment name cannot include any symbols."]
[null,"•","Sample Index rows A-D are not accessible by instrument gantry for samples ID5-ID8."]
[]
["Contact"]
[null,"support@10xgenomics.com"]
[null,"10x Genomics"]
[null,"6230 Stoneridge Mall Road"]
[null,"Pleasanton, CA 94588 USA"]
[]
[null,"© 2023 10X Genomics, Inc. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES."]

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[null,"Chromium Connect Single Cell 3' Gene Expression Input File  |  10xgenomics.com"]
[null,"After entering assay information below, click this button to autofill the LIMS file ----"]
[null,"GEX Kit","Full"]
[null,"Run Parameters","Selection","Notes (included in run logs)",null,null,"Legend"]
[null,"ExperimentName","Sample Run",null,null,null,"Blue","Enter info manually"]
[null,"Instruction Level","Standard",null,null,null,"Light Blue","Drop-down menu"]
[null,"Sample Index Plate","Dual",null,null,null,"Red","Invalid Entry"]
[null,"Sample Input Volume","10uL"]
[null,"GEM Check?","Yes"]
[null,"Mid-run QC?","Yes"]
[null,"GEX Library Const?","No"]
[null,"qPCR Setup?","No"]
[null,"Pooling?","No"]
[null,"cDNA Cycles",13]
[null,"SI Cycles",14]
[]
[null,"Number of Samples",8]
[null,"Sample Number","Sample ID","Sample Index\n(A1-H12)",null,"Cell Count","Input Type","Expression Level"]
[null,"ID1","aaa","A1",null,"500-6000","Primary Cells","High"]
[null,"ID2","bbb","B1",null,"500-6000","Primary Cells","High"]
[null,"ID3","ccc","C1",null,"500-6000","Primary Cells","High"]
[null,"ID4","ddd","D1",null,"500-6000","Primary Cells","High"]
[null,"ID5","eee","E1",null,"500-6000","Primary Cells","High"]
[null,"ID6","fff","F1",null,"500-6000","Primary Cells","High"]
[null,"ID7","ggg","G1",null,"500-6000","Primary Cells","High"]
[null,"ID8","hhh","H1",null,"500-6000","Primary Cells","High"]
[null,null,"≤ 32 characters/\nsymbols/spaces","Rows A-D not\naccessible for\nID5-ID8"]

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["Samples","Run Type","Yes/No","Cell Count","Expression","cDNA Cycles","SI Cycles","Instruction Level","SI Plate","Version","Colors","Input Type","InputVolume","InputVolumeDisplay",null,"ProtectPassword"]
[1,"Full","Yes","< 500","High",13,14,"Standard","Single","V1.34",null,"Primary Cells","StdVol","10uL",null,"10xuser"]
[2,"cDNA","No","500-6000","Low",12,12,"Advanced","Dual",null,null,"Cell Lines","HighVol","32uL"]
[3,"Library Construction",null,"> 6000",null,11,10,"Expert",null,null,null,"RNA"]
[4,null,null,null,null,null,8,null,null,null,null,"Nuclei"]
[5,null,null,null,null,null,6]
[6]
[7]
[8]
[]
[]
[]
["CSV Headers",null,null,"cDNA_volume"]
["SAMPLEPARAMETERS","SAMPLENAME","SIINDEX","VOLUME","CELLCOUNT","INPUTTYPE","EXPRESSIONLEVEL","USERDEFINED1","USERDEFINED2","USERDEFINED3","USERDEFINED4","USERDEFINED5","USERDEFINED6","USERDEFINED7","USERDEFINED8"]
["RUNPARAMETERS","SELECTION"]